RasGRP3 Antibody (H-1): sc-271068
- RasGRP3 Antibody (H-1) is a mouse monoclonal IgG2b κ RasGRP3 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-69 mapping at the N-terminus of RasGRP3 of human origin
- RasGRP3 Antibody (H-1) is recommended for detection of RasGRP3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-RasGRP3 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RasGRP3 Antibody (H-1) for WB and IHC(P) applications. These reagents are now offered in bundles with RasGRP3 Antibody (H-1) (see ordering information below).
QUICK LINKS
SEE ALSO...
RasGRP3 Antibody (H-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects RasGRP3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RasGRP3 (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RasGRP3 functions as a guanine nucleotide exchange factor (GEF), facilitating the activation of Ras and Rap1 proteins, which regulate cell proliferation, differentiation, and survival. By promoting GDP to GTP exchange, RasGRP3 connects cell surface receptors to downstream signaling pathways, particularly in prostate cancer where RasGRP3 enhances ELK1 activation, a transcription factor involved in cell growth and survival. RasGRP3 shows predominant expression in heart, brain, lung, and kidney tissues, with moderate levels in liver, skeletal muscle, pancreas, spleen, testis, and ovary, highlighting RasGRP3′s significance in various physiological processes. RasGRP3′s role in Ras activity modulation makes this protein a promising therapeutic target in diseases with dysregulated Ras signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RasGRP3 Antibody (H-1) References:
- Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 1998. DNA Res. 5: 355-64. PMID: 10048485
- Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction. | Rebhun, JF., et al. 2000. J Biol Chem. 275: 34901-8. PMID: 10934204
- Activation of RasGRP3 by phosphorylation of Thr-133 is required for B cell receptor-mediated Ras activation. | Aiba, Y., et al. 2004. Proc Natl Acad Sci U S A. 101: 16612-7. PMID: 15545601
- A vascular gene trap screen defines RasGRP3 as an angiogenesis-regulated gene required for the endothelial response to phorbol esters. | Roberts, DM., et al. 2004. Mol Cell Biol. 24: 10515-28. PMID: 15572660
- Phosphorylation of RasGRP3 on threonine 133 provides a mechanistic link between PKC and Ras signaling systems in B cells. | Zheng, Y., et al. 2005. Blood. 105: 3648-54. PMID: 15657177
- Role of phorbol ester localization in determining protein kinase C or RasGRP3 translocation: real-time analysis using fluorescent ligands and proteins. | Braun, DC., et al. 2005. Mol Cancer Ther. 4: 141-50. PMID: 15657361
- RasGRP3 mediates phorbol ester-induced, protein kinase C-independent exocytosis. | Ozaki, N., et al. 2005. Biochem Biophys Res Commun. 329: 765-71. PMID: 15737652
- Diacylglycerol kinase iota regulates Ras guanyl-releasing protein 3 and inhibits Rap1 signaling. | Regier, DS., et al. 2005. Proc Natl Acad Sci U S A. 102: 7595-600. PMID: 15894621
- RasGRP1 and RasGRP3 regulate B cell proliferation by facilitating B cell receptor-Ras signaling. | Coughlin, JJ., et al. 2005. J Immunol. 175: 7179-84. PMID: 16301621
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RasGRP3 Antibody (H-1) | sc-271068 | 200 µg/ml | $322.00 | |||
RasGRP3 Antibody (H-1): m-IgG Fc BP-HRP Bundle | sc-529114 | 200 µg Ab; 10 µg BP | $361.00 | |||
RasGRP3 Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-521839 | 200 µg Ab, 40 µg BP | $361.00 | |||
RasGRP3 Antibody (H-1): m-IgG2b BP-HRP Bundle | sc-550081 | 200 µg Ab; 10 µg BP | $361.00 | |||
RasGRP3 Antibody (H-1) AC | sc-271068 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RasGRP3 Antibody (H-1) HRP | sc-271068 HRP | 200 µg/ml | $322.00 | |||
RasGRP3 Antibody (H-1) FITC | sc-271068 FITC | 200 µg/ml | $336.00 | |||
RasGRP3 Antibody (H-1) PE | sc-271068 PE | 200 µg/ml | $349.00 | |||
RasGRP3 Antibody (H-1) Alexa Fluor® 488 | sc-271068 AF488 | 200 µg/ml | $364.00 | |||
RasGRP3 Antibody (H-1) Alexa Fluor® 546 | sc-271068 AF546 | 200 µg/ml | $364.00 | |||
RasGRP3 Antibody (H-1) Alexa Fluor® 594 | sc-271068 AF594 | 200 µg/ml | $364.00 | |||
RasGRP3 Antibody (H-1) Alexa Fluor® 647 | sc-271068 AF647 | 200 µg/ml | $364.00 | |||
RasGRP3 Antibody (H-1) Alexa Fluor® 680 | sc-271068 AF680 | 200 µg/ml | $364.00 | |||
RasGRP3 Antibody (H-1) Alexa Fluor® 790 | sc-271068 AF790 | 200 µg/ml | $364.00 |