Ral A Antibody (F-5): sc-374462

Ral A Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects RalA of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RalA antibody (F-5) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RalA is a member of the Ras-related GTPase family, playing a crucial role in cellular signaling pathways. RalA regulates exocytosis and cytoskeletal dynamics, making RalA essential for cell migration and proliferation. RalA primarily localizes in the cytoplasm and can move to the plasma membrane upon activation, which drives signal transduction. RalA becomes activated by guanine nucleotide exchange factor RalGDS and inactivated by specific GTPase-activating proteins, ensuring precise signaling control. Unlike other Ras proteins, RalA doesn′t induce transformed foci, highlighting a unique role in cellular processes. RalA interacts with downstream effectors, including RalBP-1, which contains a Rho-GTPase-activating domain preferentially interacting with Cdc42, a Rho family member. This interaction showcases RalA′s role in modulating various signaling pathways, including phospholipase D activation, emphasizing RalA′s significance in cellular function and potential cancer biology implications.

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Ral A Antibody (F-5) References:

1. A Ral GAP complex links PI 3-kinase/Akt signaling to RalA activation in insulin action.  |  Chen, XW., et al. 2011. Mol Biol Cell. 22: 141-52. PMID: 21148297
2. Systematic pan-cancer analysis identifies RALA as a tumor targeting immune therapeutic and prognostic marker.  |  Jin, H., et al. 2022. Front Immunol. 13: 1046044. PMID: 36466919
3. Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module.  |  Wang, S., et al. 2023. Sci Adv. 9: eade2540. PMID: 36812304
4. Identification of RALA as a Therapeutic Target and Prognostic Predictor of Osteosarcoma.  |  Fan, G., et al. 2023. Biomed Res Int. 2023: 1150768. PMID: 36817861
5. A RalA between high-fat diet and mitochondrial shape.  |  Zambello, L. and Scorrano, L. 2024. Nat Metab. 6: 198-199. PMID: 38286820
6. Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation.  |  Xia, W., et al. 2024. Nat Metab. 6: 273-289. PMID: 38286821
7. Involvement of Ral GTPase in v-Src-induced phospholipase D activation.  |  Jiang, H., et al. 1995. Nature. 378: 409-12. PMID: 7477381
8. Identification and characterization of Ral-binding protein 1, a potential downstream target of Ral GTPases.  |  Cantor, SB., et al. 1995. Mol Cell Biol. 15: 4578-84. PMID: 7623849
9. Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity.  |  Jullien-Flores, V., et al. 1995. J Biol Chem. 270: 22473-7. PMID: 7673236
10. Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins.  |  Wildey, GM., et al. 1993. Biochem Biophys Res Commun. 194: 552-9. PMID: 7687439
11. Identification of the guanine nucleotide dissociation stimulator for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and Rap.  |  Spaargaren, M. and Bischoff, JR. 1994. Proc Natl Acad Sci U S A. 91: 12609-13. PMID: 7809086
12. Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator.  |  Hofer, F., et al. 1994. Proc Natl Acad Sci U S A. 91: 11089-93. PMID: 7972015

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ral A Antibody (F-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Ral A Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ral A Antibody (F-5) Specifications

Alternate Product Name: Ral A

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 181-209

Epitope Target: Ral A

Epitope Species: human

Product Reactivity/Detection: Ral A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Ral A Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin