Rac 1/2/3 Antibody (G-2): sc-514583

Rac 1/2/3 Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect Rac1, Rac2, and Rac3 proteins from mouse, rat, and human origins across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Rac 1/2/3 (G-2) monoclonal antibody targets a precise epitope mapping between amino acids 163-183 near the C-terminus of human Rac1, ensuring robust and reliable binding for diverse experimental needs. Rac proteins are integral members of the Ras superfamily of small GTPases, which are pivotal in regulating a multitude of fundamental cellular processes such as cell growth, vesicle trafficking, and cytoskeletal organization. Specifically, Rac1 is ubiquitously expressed in numerous cell types and plays a crucial role in controlling cell morphology, migration, and proliferation by modulating actin cytoskeleton dynamics. Rac2, predominantly expressed in myeloid cells, is essential for the regulation of the NADPH oxidase complex in neutrophils, thereby contributing significantly to the immune response and reactive oxygen species production. Rac 1/2/3 (G-2) antibody is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different research applications. By targeting these key regulatory proteins, anti-Rac 1/2/3 (G-2) antibody facilitates the investigation of critical signaling pathways involved in cellular dynamics, immune function, and related pathological conditions, making this tool indispensable for researchers aiming to elucidate the molecular mechanisms underlying health and disease.

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Rac 1/2/3 Antibody (G-2) References:

1. Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac 2.  |  Knaus, UG., et al. 1991. Science. 254: 1512-5. PMID: 1660188
2. Bcr encodes a GTPase-activating protein for p21rac.  |  Diekmann, D., et al. 1991. Nature. 351: 400-2. PMID: 1903516
3. The cellular functions of small GTP-binding proteins.  |  Hall, A. 1990. Science. 249: 635-40. PMID: 2116664
4. The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae.  |  Xu, GF., et al. 1990. Cell. 63: 835-41. PMID: 2121369
5. The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21.  |  Martin, GA., et al. 1990. Cell. 63: 843-9. PMID: 2121370
6. The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins.  |  Ballester, R., et al. 1990. Cell. 63: 851-9. PMID: 2121371
7. rac, a novel ras-related family of proteins that are botulinum toxin substrates.  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
8. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants.  |  Trahey, M. and McCormick, F. 1987. Science. 238: 542-5. PMID: 2821624
9. Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins.  |  Sewell, JL. and Kahn, RA. 1988. Proc Natl Acad Sci U S A. 85: 4620-4. PMID: 3133654

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rac 1/2/3 Antibody (G-2) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Rac 1/2/3 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rac 1/2/3 Antibody (G-2) Specifications

Alternate Product Name: Rac 1/2/3

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 163-183

Epitope Target: Rac 1

Epitope Species: human

Product Reactivity/Detection: Rac 1, Rac 2 and Rac 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Rac 1/2/3 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin