R1 Antibody (E-7): sc-377426
- R1 Antibody (E-7) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-300 of R1 of human origin
- recommended for detection of R1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for R1 Antibody (E-7). This work is in progress.
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R1 Antibody (E-7) is a mouse monoclonal IgG2b antibody that detects ribonucleotide reductase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ribonucleotide reductase antibody (E-7) specifically targets the R1 subunit of ribonucleotide reductase, which plays a crucial role in the synthesis of deoxyribonucleotide triphosphates (dNTPs), the building blocks necessary for DNA replication and repair. The R1 subunit is notable for allosteric regulatory sites, which allow responses to cellular signals and modulate enzyme activity, thereby ensuring appropriate dNTP levels during the cell cycle. This regulation supports the fidelity of DNA synthesis and repair processes, vital for maintaining genomic stability. Additionally, ribonucleotide reductase participates in nucleotide excision repair, with both R1 and R2 subunits being induced in response to UV light, highlighting their role in cellular responses to DNA damage. Anti-ribonucleotide reductase antibody (E-7) effectively detects this essential protein, making R1 (E-7) monoclonal antibody an invaluable tool for researchers studying DNA synthesis, repair mechanisms, and cell cycle regulation.
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R1 Antibody (E-7) References:
- The U28 ORF of human herpesvirus-7 does not encode a functional ribonucleotide reductase R1 subunit. | Sun, Y. and Conner, J. 1999. J Gen Virol. 80 (Pt 10): 2713-2718. PMID: 10573165
- A polyethylenimine-linoleic acid conjugate for antisense oligonucleotide delivery. | Xie, J., et al. 2013. Biomed Res Int. 2013: 710502. PMID: 23862153
- Microfluidic assembly of lipid nanoparticles for delivery of antisense oligonucleotides. | Zhou, Y., et al. 2014. Curr Pharm Biotechnol. 15: 823-8. PMID: 25335534
- Enhanced antisense oligonucleotide delivery using cationic liposomes incorporating fatty acid-modified polyethylenimine. | Guo, Z., et al. 2014. Curr Pharm Biotechnol. 15: 800-5. PMID: 25403516
- A novel reduction-sensitive modified polyethylenimine oligonucleotide vector. | Yang, S., et al. 2015. Int J Pharm. 484: 44-50. PMID: 25698089
- Non-covalent complexes of folic acid and oleic acid conjugated polyethylenimine: An efficient vehicle for antisense oligonucleotide delivery. | Yang, S., et al. 2015. Colloids Surf B Biointerfaces. 135: 274-282. PMID: 26263216
- NMR structure of an inhibitory R2 C-terminal peptide bound to mouse ribonucleotide reductase R1 subunit. | Fisher, A., et al. 1995. Nat Struct Biol. 2: 951-5. PMID: 7583667
- Affinity of synthetic peptides for the HSV-2 ribonucleotide reductase R1 subunit measured with an iodinated photoaffinity peptide. | Lamarche, N., et al. 1994. Anal Biochem. 220: 315-20. PMID: 7978273
- Purification, characterization, and localization of subunit interaction area of recombinant mouse ribonucleotide reductase R1 subunit. | Davis, R., et al. 1994. J Biol Chem. 269: 23171-6. PMID: 8083221
- c-Myc overexpression associated DHFR gene amplification in hamster, rat, mouse and human cell lines. | Mai, S., et al. 1996. Oncogene. 12: 277-88. PMID: 8570205
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
R1 Antibody (E-7) | sc-377426 | 200 µg/ml | $316.00 |