QTRT1 Antibody (D-7): sc-398918
- QTRT1 Antibody (D-7) is a mouse monoclonal IgG2b κ QTRT1 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 111-136 within an internal region of QTRT1 of human origin
- QTRT1 Antibody (D-7) is recommended for detection of QTRT1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-QTRT1 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for QTRT1 Antibody (D-7) for WB applications. These reagents are now offered in bundles with QTRT1 Antibody (D-7) (see ordering information below).
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QTRT1 Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects QTRT1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-QTRT1 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. QTRT1, or queuine tRNA-ribosyltransferase 1, consists of 403 amino acids and is a member of the queuine tRNA-ribosyltransferase family, playing a crucial role in the modification of tRNA molecules. Specifically, QTRT1 catalyzes the exchange of queuine for guanine at the wobble position of tRNAs with GUN anticodons, resulting in the formation of the hypermodified nucleoside queuosine, which is essential for proper tRNA functioning and protein synthesis. The interaction between QTRT1 and QTRTD1 is vital for enzymatic activity, as both work together to facilitate this modification. QTRT1 contains four key residues—Cys302, Cys304, Cys307, and His333—that are critical for zinc ion binding, with one zinc ion bound per subunit, highlighting the importance of metal ions in enzymatic function. QTRT1 is highly conserved across various species, including chimpanzees, dogs, cows, zebrafish, and even model organisms like fruit flies and C. elegans, and is located on human chromosome 19p13.2, underscoring evolutionary significance and potential implications in genetic studies and disease research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
QTRT1 Antibody (D-7) References:
- Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit. | Deshpande, KL. and Katze, JR. 2001. Gene. 265: 205-12. PMID: 11255023
- The DNA sequence and biology of human chromosome 19. | Grimwood, J., et al. 2004. Nature. 428: 529-35. PMID: 15057824
- Queuosine formation in eukaryotic tRNA occurs via a mitochondria-localized heteromeric transglycosylase. | Boland, C., et al. 2009. J Biol Chem. 284: 18218-27. PMID: 19414587
- Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. | Chen, YC., et al. 2010. RNA. 16: 958-68. PMID: 20354154
- Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases. | Chen, YC., et al. 2011. Nucleic Acids Res. 39: 2834-44. PMID: 21131277
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QTRT1 Antibody (D-7) | sc-398918 | 200 µg/ml | $316.00 | |||
QTRT1 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-530969 | 200 µg Ab; 10 µg BP | $354.00 | |||
QTRT1 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-524536 | 200 µg Ab, 40 µg BP | $354.00 | |||
QTRT1 Antibody (D-7): m-IgG2b BP-HRP Bundle | sc-550245 | 200 µg Ab; 10 µg BP | $354.00 | |||
QTRT1 Antibody (D-7) AC | sc-398918 AC | 500 µg/ml, 25% agarose | $416.00 | |||
QTRT1 Antibody (D-7) HRP | sc-398918 HRP | 200 µg/ml | $316.00 | |||
QTRT1 Antibody (D-7) FITC | sc-398918 FITC | 200 µg/ml | $330.00 | |||
QTRT1 Antibody (D-7) PE | sc-398918 PE | 200 µg/ml | $343.00 | |||
QTRT1 Antibody (D-7) Alexa Fluor® 488 | sc-398918 AF488 | 200 µg/ml | $357.00 | |||
QTRT1 Antibody (D-7) Alexa Fluor® 546 | sc-398918 AF546 | 200 µg/ml | $357.00 | |||
QTRT1 Antibody (D-7) Alexa Fluor® 594 | sc-398918 AF594 | 200 µg/ml | $357.00 | |||
QTRT1 Antibody (D-7) Alexa Fluor® 647 | sc-398918 AF647 | 200 µg/ml | $357.00 | |||
QTRT1 Antibody (D-7) Alexa Fluor® 680 | sc-398918 AF680 | 200 µg/ml | $357.00 | |||
QTRT1 Antibody (D-7) Alexa Fluor® 790 | sc-398918 AF790 | 200 µg/ml | $357.00 | |||
QTRT1 (D-7) Neutralizing Peptide | sc-398918 P | 100 µg/0.5 ml | $68.00 |