PTP1B Antibody (H-9) recommended for detection of PTP1B of human origin by WB, IP, IF, IHC(P) and ELISA

PTP1B Antibody (H-9): sc-133258

PTP1B Antibody (H-9) is rated 5.0 out of 5 by 5.
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Datasheets

    • PTP1B Antibody (H-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 301-435 mapping at the C-terminus of PTP1B (protein tyrosine phosphatase) of human origin
    • recommended for detection of PTP1B of human origin by WB, IP, IF, IHC(P) and ELISA
    • See PTP1B (D-4): sc-133259 for PTP1B antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using PTP1B (H-9): sc-133258 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice antibody for western blot bands We used this antibody for western blot in order to detect PTP1B expression. We got positive bands shown up at the expected molecular weight in Jurkat cells.
Date published: 2017-01-27
Rated 5 out of 5 by from Strong band seen in WB in HL Strong band seen in WB in HL-60 whole cell lysate. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Good antibody for WB in JAR whole cell lysate Good antibody for WB in JAR whole cell lysate. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Positive band in WB in human PTP1B transfected Positive band in WB in human PTP1B transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-01-15
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