Anti-PTP1B Antibody (D-4) recommended for detection of PTP1B of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PTP1B Antibody (D-4): sc-133259

Anti-PTP1B Antibody (D-4) is rated 4.2 out of 5 by 13.
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Datasheets

    • Anti-PTP1B Antibody (D-4) is a mouse monoclonal IgG1 κ PTP1B antibody, cited in 11 publications, provided at 200 µg/ml
    • raised against amino acids 301-435 mapping at the C-terminus of PTP1B (protein tyrosine phosphatase) of human origin
    • PTP1B Antibody (D-4) is recommended for detection of PTP1B of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PTP1B Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PTP1B (D-4): sc-133259.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PTP1B Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with PTP1B Antibody (D-4) (see ordering information below).
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Ist der Antikörper reversibel oder irreversibel an die Agarose gebunden?

Asked by: Marienfeld
Vielen Dank für Ihre Anfrage und Ihr Interesse an diesem Produkt. Der als Agarose-Konjugat erhältliche PTP1B Antikörper (D-4): sc-133259 AC ist kovalent an Agarose-Beads über primäre Amine gebunden. Für weitere Informationen wenden Sie sich bitte an unseren Technischen Service in unserem Europäischen Supportbüro in Heidelberg. Sie erreichen uns telefonisch unter 06221 4503 0, per email an: [email protected] oder per Live-Chat auf unserer Webseite www.scbt.com Wir freuen uns Ihnen weiterzuhelfen.
Answered by: Technical Support Europe
Date published: 2017-05-22

Can PTP1B (D-4): sc-133259 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PTP1B (D-4): sc-133259 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IHC Our lab usually use this antibody staining PTP1B expression in Human liver cancer tissue section by IHC.We usuallly use goat serum for blocking and 1;100 dilution .We use ABC kit from Vector Labs.
Date published: 2018-05-26
Rated 4 out of 5 by from Very good results with western blots very good result (single sharp band with expected size) with HUVECS lysates.
Date published: 2018-01-26
Rated 4 out of 5 by from Fairly good! Although there is a non-specific band in some cell lysates, the signal is strong and clean.
Date published: 2017-11-15
Rated 5 out of 5 by from Great I could detect one strong band using HEK293 whole cell lysate.
Date published: 2017-10-13
Rated 4 out of 5 by from Western blot I tested with PTP1B(D-4), SC133259 for Western blot in 293 cotransfection. This antibody works well.
Date published: 2017-05-10
Rated 3 out of 5 by from not worked in rat cells I tried this antibody in L6 cells, but it not worked. No band can be detected.
Date published: 2017-05-10
Rated 4 out of 5 by from woking well I used this antibody for Western blot with 293 cotransfected cell lysate. I can detect a clear thick band with expected size even though 293 cells express low amount of endogenous PTP1B.
Date published: 2017-04-20
Rated 5 out of 5 by from Strong and robuts PTP1B Antibody (D-4) produced strong and robust results by Western blot and IHCP
Date published: 2017-01-27
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