PTP1B Antibody (D-4): sc-133259

PTP1B Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PTP1B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-PTP1B antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PTP1B plays a crucial role in regulating insulin signaling and is involved in the negative regulation of the insulin receptor, making PTP1B a significant target for understanding metabolic disorders such as obesity and diabetes. Located primarily in the endoplasmic reticulum and cytoplasm, PTP1B′s positioning is essential for function, as PTP1B interacts with various signaling pathways that modulate cellular responses to insulin. PTP1B is synthesized as a 435 amino acid precursor, which is subsequently cleaved to produce the active 321 amino acid form, highlighting the importance of post-translational modifications in activation and function. With unique structural characteristics and regulatory roles, PTP1B serves as a vital component in cellular signaling, making PTP1B monoclonal antibody (D-4) an invaluable tool for researchers studying insulin resistance and related metabolic conditions.

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PTP1B Antibody (D-4) References:

1. Cloning of a cDNA for a major human protein-tyrosine-phosphatase.  |  Chernoff, J., et al. 1990. Proc Natl Acad Sci U S A. 87: 2735-9. PMID: 2157211
2. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
3. Phosphotyrosyl protein phosphatases.  |  Lau, KH., et al. 1989. Biochem J. 257: 23-36. PMID: 2537623
4. Human placenta protein-tyrosine-phosphatase: amino acid sequence and relationship to a family of receptor-like proteins.  |  Charbonneau, H., et al. 1989. Proc Natl Acad Sci U S A. 86: 5252-6. PMID: 2546149
5. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.  |  Gould, KL. and Nurse, P. 1989. Nature. 342: 39-45. PMID: 2682257
6. Purification of the major protein-tyrosine-phosphatases of human placenta.  |  Tonks, NK., et al. 1988. J Biol Chem. 263: 6722-30. PMID: 2834386
7. A new member of the immunoglobulin superfamily that has a cytoplasmic region homologous to the leukocyte common antigen.  |  Streuli, M., et al. 1988. J Exp Med. 168: 1523-30. PMID: 2972792
8. Protein-tyrosine kinases.  |  Hunter, T. and Cooper, JA. 1985. Annu Rev Biochem. 54: 897-930. PMID: 2992362
9. Regulation of PTP1B activation through disruption of redox-complex formation.  |  Londhe, AD., et al. 2020. Nat Chem Biol. 16: 122-125. PMID: 31873221
10. PTP1B negatively regulates STAT1-independent Pseudomonas aeruginosa killing by macrophages.  |  Yue, L., et al. 2020. Biochem Biophys Res Commun. 533: 296-303. PMID: 32958258
11. Protein tyrosine phosphatase 1B(PTP1B) promotes melanoma cells progression through Src activation.  |  Wang, Q., et al. 2021. Bioengineered. 12: 8396-8406. PMID: 34606417
12. Discovery of Novel PTP1B Inhibitors by High-throughput Virtual Screening.  |  Debnath, A., et al. 2024. Curr Comput Aided Drug Des.. PMID: 39411941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PTP1B Antibody (D-4) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-PTP1B Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PTP1B Antibody (D-4) Specifications

Alternate Product Name: PTP1B

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 301-435

Epitope Target: PTP1B (protein tyrosine phosphatase)

Epitope Species: human

Product Reactivity/Detection: PTP1B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for PTP1B Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin