PTP IA-2β Antibody (B-4): sc-393922
- PTP IA-2β Antibody (B-4) is a mouse monoclonal IgG2a κ PTP IA-2β antibody provided at 200 µg/ml
- raised against amino acids 61-360 mapping near the N-terminus of PTP IA-2β of human origin
- PTP IA-2 beta Antibody (B-4) is recommended for detection of PTP IA-2β of human origin by WB, IP, IF and ELISA
- Anti-PTP IA-2 beta Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PTP IA-2β Antibody (B-4) for WB applications. These reagents are now offered in bundles with PTP IA-2β Antibody (B-4) (see ordering information below).
PTP IA-2β Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects PTP IA-2 beta protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PTP IA-2β (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Protein-Tyrosine Phosphatase Receptor-Type IA-2β (PTP IA-2β), also known as PTPRN2 or Phogrin, plays a crucial role in regulating insulin secretion as PTP IA-2β is localized in dense-core secretory vesicles of pancreatic islet cells. This localization allows PTP IA-2β to modulate signaling pathways involved in insulin release, thereby influencing glucose homeostasis. PTP IA-2β serves as an autoantigen associated with insulin-dependent diabetes mellitus (IDDM), with autoantigenic epitopes residing within the cytoplasmic domain of this transmembrane protein. The PTPRN2 gene, which encodes PTP IA-2β, is located on chromosome 7q36 and is predominantly expressed in human brain and pancreas, as evidenced by Northern blot analysis revealing 5.5- and 3.7-kb transcripts. Three alternative splice variants of PTPRN2 produce distinct proteins, further underscoring the complexity and significance of PTP IA-2β in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PTP IA-2β Antibody (B-4) References:
- Multimerization of the protein-tyrosine phosphatase (PTP)-like insulin-dependent diabetes mellitus autoantigens IA-2 and IA-2beta with receptor PTPs (RPTPs). Inhibition of RPTPalpha enzymatic activity. | Gross, S., et al. 2002. J Biol Chem. 277: 48139-45. PMID: 12364328
- Spontaneous peripheral T-cell responses to the IA-2beta (phogrin) autoantigen in young nonobese diabetic mice. | Achenbach, P., et al. 2002. J Autoimmun. 19: 111-6. PMID: 12419281
- A novel strategy for the development of selective active-site inhibitors of the protein tyrosine phosphatase-like proteins islet-cell antigen 512 (IA-2) and phogrin (IA-2beta). | Drake, PG., et al. 2003. Biochem J. 373: 393-401. PMID: 12697028
- Targeted disruption of the IA-2beta gene causes glucose intolerance and impairs insulin secretion but does not prevent the development of diabetes in NOD mice. | Kubosaki, A., et al. 2004. Diabetes. 53: 1684-91. PMID: 15220191
- Molecular cloning and characterization of the human transmembrane protein tyrosine phosphatase homologue, phogrin, an autoantigen of type 1 diabetes. | Kawasaki, E., et al. 1996. Biochem Biophys Res Commun. 227: 440-7. PMID: 8878534
- ICAAR, a novel member of a new family of transmembrane, tyrosine phosphatase-like proteins. | Smith, PD., et al. 1996. Biochem Biophys Res Commun. 229: 402-11. PMID: 8954911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTP IA-2β Antibody (B-4) | sc-393922 | 200 µg/ml | $316.00 | |||
PTP IA-2β Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-526273 | 200 µg Ab; 10 µg BP | $354.00 | |||
PTP IA-2β Antibody (B-4): m-IgG2a BP-HRP Bundle | sc-546553 | 200 µg Ab; 10 µg BP | $354.00 | |||
PTP IA-2β Antibody (B-4) AC | sc-393922 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PTP IA-2β Antibody (B-4) HRP | sc-393922 HRP | 200 µg/ml | $316.00 | |||
PTP IA-2β Antibody (B-4) FITC | sc-393922 FITC | 200 µg/ml | $330.00 | |||
PTP IA-2β Antibody (B-4) PE | sc-393922 PE | 200 µg/ml | $343.00 | |||
PTP IA-2β Antibody (B-4) Alexa Fluor® 488 | sc-393922 AF488 | 200 µg/ml | $357.00 | |||
PTP IA-2β Antibody (B-4) Alexa Fluor® 546 | sc-393922 AF546 | 200 µg/ml | $357.00 | |||
PTP IA-2β Antibody (B-4) Alexa Fluor® 594 | sc-393922 AF594 | 200 µg/ml | $357.00 | |||
PTP IA-2β Antibody (B-4) Alexa Fluor® 647 | sc-393922 AF647 | 200 µg/ml | $357.00 | |||
PTP IA-2β Antibody (B-4) Alexa Fluor® 680 | sc-393922 AF680 | 200 µg/ml | $357.00 | |||
PTP IA-2β Antibody (B-4) Alexa Fluor® 790 | sc-393922 AF790 | 200 µg/ml | $357.00 |