PR Antibody (F-4) recommended for detection of PR-A and PR-B of human origin by WB, IP, IF, IHC(P) and ELISA

PR Antibody (F-4): sc-166169

PR Antibody (F-4) is rated 4.2 out of 5 by 19.
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Datasheets

    • Anti-PR Antibody (F-4) is a mouse monoclonal IgG1 (kappa light chain) PR antibody provided at 200 µg/ml
    • raised against amino acids 375-564 of PR of human origin
    • PR Antibody (F-4) is recommended for detection of PR-A and PR-B of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PR Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-166169 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PR (F-4): sc-166169.
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Hi, Do you know the antigen epitope for F4 PR antibody? This information is important for us.

Asked by: VValerie
Thank you for your question. This antibody was raised against an antigen corresponding to amino acids 375-564 of PR of human origin. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-05-03

Hi! Would it be possible to receive a free 10 µg sample of PR (F-4): sc-166169 . Thank you very much for your availability, have a nice day! Best regards, Fabio De Martino.

Asked by: fdemarti
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

When should the TransCruz reagent sc-166169 X be used instead of the standard form sc-166169?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not for ChIP-qPCR Used 4ug of this antibody in an optimized protocol for ChIP-qPCR in T-47D and ZR.75.1 cell lines and it didn't work.
Date published: 2020-02-17
Rated 5 out of 5 by from IHC excellent results Strong immunostaining signal in paraffin sections of myometrium and fibroids with no background.
Date published: 2018-07-06
Rated 5 out of 5 by from Excelente para IHC Recibimos este anticuerpo para testar y lo hemos probado para IHC en muestras humanas (PDX) de parafina. Funciona tanto a pH9 como pH6, con un resultado ligeramente superior a pH9 en el retrival.
Date published: 2018-04-30
Rated 5 out of 5 by from PR antibody Got sample of this. Worked very well for WB and for IF. Will detect both A and B isoforms ; can depend on cell line.
Date published: 2018-03-05
Rated 5 out of 5 by from Clear Signal I am happy with my ICC result. It is clear with no background.
Date published: 2018-02-27
Rated 5 out of 5 by from Ishikawa細胞ではきれいにでます。 Ishikawa細胞のタンパクを10μg applyしました。比較的きれいにバンドを出すことができました。
Date published: 2018-02-23
Rated 4 out of 5 by from test en cytométrie de flux j'ai demandé un échantillon gratuit pour tester l'anticorps dans mon modèle pour la cytométrie de flux, j'ai obtenu un très bon marquage sans bruit de fond.
Date published: 2018-02-15
Rated 4 out of 5 by from works well I was testing this antibody on my human cell lines. Works for IF, no background.
Date published: 2017-11-23
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