Progesterone Receptor Antibody (AB-52): sc-810

PR Antibody (AB-52) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect both PR-A and PR-B isoforms of the progesterone receptor across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC) applications. PR monoclonal antibody (AB-52) is produced by immunizing with native human progesterone receptor purified from breast cancer cells, ensuring robust affinity and specificity for accurate detection. Progesterone receptor (PR) is a vital nuclear hormone receptor located within the cell nucleus, where PR functions as a transcription factor to regulate gene expression in response to progesterone binding. Nuclear localization is crucial for PR′s role in controlling essential biological processes such as the menstrual cycle, maintenance of pregnancy, and development of reproductive tissues. PR exists in two main isoforms, PR-A and PR-B, which are transcribed from distinct promoters and exhibit different functional properties. PR-B primarily acts as a transcriptional activator, promoting expression of genes involved in cell growth, differentiation, and apoptosis, while PR-A functions as a transcriptional repressor, modulating activity of other steroid hormone receptors. PR monoclonal antibody (AB-52) is recommended for investigating progesterone signaling pathways, receptor dynamics, and implications in health and disease, thereby facilitating a deeper understanding of molecular mechanisms underlying hormone action and potential therapeutic targets.

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Progesterone Receptor Antibody (AB-52) References:

1. The A and B isoforms of the human progesterone receptor: two functionally different transcription factors encoded by a single gene.  |  Giangrande, PH. and McDonnell, DP. 1999. Recent Prog Horm Res. 54: 291-313; discussion 313-4. PMID: 10548881
2. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody.  |  Clemm, DL., et al. 2000. Mol Endocrinol. 14: 52-65. PMID: 10628747
3. The opposing transcriptional activities of the two isoforms of the human progesterone receptor are due to differential cofactor binding.  |  Giangrande, PH., et al. 2000. Mol Cell Biol. 20: 3102-15. PMID: 10757795
4. Reproductive functions of progesterone receptors.  |  Conneely, OM., et al. 2002. Recent Prog Horm Res. 57: 339-55. PMID: 12017551
5. Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B.  |  Kastner, P., et al. 1990. EMBO J. 9: 1603-14. PMID: 2328727
6. The progesterone receptor gene maps to human chromosome band 11q13, the site of the mammary oncogene int-2.  |  Law, ML., et al. 1987. Proc Natl Acad Sci U S A. 84: 2877-81. PMID: 3472240
7. The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells.  |  Wen, DX., et al. 1994. Mol Cell Biol. 14: 8356-64. PMID: 7969170
8. Stoichiometry and site-specific phosphorylation of human progesterone receptor in native target cells and in the baculovirus expression system.  |  Beck, CA., et al. 1996. J Biol Chem. 271: 19546-55. PMID: 8702648
9. Phosphorylation of human progesterone receptor by cyclin-dependent kinase 2 on three sites that are authentic basal phosphorylation sites in vivo.  |  Zhang, Y., et al. 1997. Mol Endocrinol. 11: 823-32. PMID: 9171245
10. Mapping and characterization of the functional domains responsible for the differential activity of the A and B isoforms of the human progesterone receptor.  |  Giangrande, PH., et al. 1997. J Biol Chem. 272: 32889-900. PMID: 9407067

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Progesterone Receptor Antibody (AB-52) has 81 citations in a variety of scientific publications.

Primary antibodies like Anti-Progesterone Receptor Antibody (AB-52) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Progesterone Receptor Antibody (AB-52) Specifications

Alternate Product Name: progesterone receptor

Product Clone Name: AB-52

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PR

Epitope Species: human

Product Reactivity/Detection: PR-A and PR-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 81 citations in various scientific publications

Available Conjugates/Forms for Progesterone Receptor Antibody (AB-52): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, X: TransCruz Reagent