Progesterone Receptor Antibody (F-2): sc-166170

PR Antibody (F-2) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect both PR-A and PR-B isoforms of human progesterone receptor across multiple species, including mouse, rat, and human, with exceptional accuracy in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-progesterone receptor antibody (F-2) is meticulously raised against amino acids 375-564 of human progesterone receptor, targeting a crucial region that facilitates interactions with various co-regulatory proteins essential for receptor function. Progesterone receptor (PR) is a sophisticated nuclear hormone receptor that plays a pivotal role in mediating progesterone effects by regulating gene transcription. PR exists in two main isoforms, PR-A and PR-B, which are transcribed from distinct, estrogen-inducible promoters within a single PR gene. PR-B contains an additional 164 amino acids at its N-terminus compared to PR-A, resulting in different transcriptional activation properties; while PR-B typically acts as a potent transcriptional activator, PR-A functions as a ligand-dependent transdominant repressor of other steroid hormone receptors. These isoforms interact with a variety of coactivators and corepressors, such as SRC-1, NCoA1, and NCoR, which modulate transcriptional activity and specificity, thereby influencing cellular processes like proliferation, differentiation, and apoptosis. Both PR-A and PR-B contain an inhibitory domain in their amino terminus, which is functionally active only in PR-A and is necessary for transrepression of steroid hormone signaling, highlighting distinct conformations and regulatory mechanisms of each isoform within cells. Progesterone receptor monoclonal antibody (F-2) is an invaluable tool for researchers aiming to elucidate complex signaling pathways and structural-functional dynamics of progesterone receptor, thereby advancing understanding of progesterone′s role in various physiological and pathological contexts.

The preferred Progesterone Receptor antibody is Progesterone Receptor Antibody (AB-52): sc-810.