Polycystin-1 Antibody (7E12): sc-130554

Polycystin-1 Antibody (7E12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Polycystin-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Polycystin-1 monoclonal antibody (7E12) is raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin. Polycystin-1 (7E12) antibody is available in both non-conjugated and various conjugated forms, including agarose, phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Polycystin-1 plays a crucial role in regulating cell proliferation and polarity, which is particularly important in autosomal dominant polycystic kidney disease (ADPKD), a condition characterized by cyst formation in kidney tubules and other organs. Mutations in Polycystin-1 are implicated in most ADPKD cases, leading to abnormal cellular behavior within cysts. Polycystin-1 primarily localizes in renal tubular epithelial cells, where Polycystin-1 colocalizes with cell adhesion proteins such as E-cadherin, catenins, vinculin, and paxillin, forming a multiprotein complex essential for maintaining tissue architecture and function. Additionally, Polycystin-1 undergoes post-translational modifications, including tyrosine phosphorylation, which enhances interaction with Polycystin-2 (PKD2) and suggests a role in mediating AP-1 expression. These interactions highlight Polycystin-1′s importance in cell-cell and cell-matrix communication, ultimately influencing cellular responses vital for kidney function and overall homeostasis.

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Polycystin-1 Antibody (7E12) References:

1. Cellular activation triggered by the autosomal dominant polycystic kidney disease gene product PKD2.  |  Arnould, T., et al. 1999. Mol Cell Biol. 19: 3423-34. PMID: 10207066
2. Coordinate expression of the autosomal dominant polycystic kidney disease proteins, polycystin-2 and polycystin-1, in normal and cystic tissue.  |  Ong, AC., et al. 1999. Am J Pathol. 154: 1721-9. PMID: 10362797
3. Polycystin-1 expression in PKD1, early-onset PKD1, and TSC2/PKD1 cystic tissue.  |  Ong, AC., et al. 1999. Kidney Int. 56: 1324-33. PMID: 10504485
4. The PKD1 gene product, 'polycystin-1,' is a tyrosine-phosphorylated protein that colocalizes with alpha2beta1-integrin in focal clusters in adherent renal epithelia.  |  Wilson, PD., et al. 1999. Lab Invest. 79: 1311-23. PMID: 10532593
5. Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins.  |  Huan, Y. and van Adelsberg, J. 1999. J Clin Invest. 104: 1459-68. PMID: 10562308
6. Identification, characterization, and localization of a novel kidney polycystin-1-polycystin-2 complex.  |  Newby, LJ., et al. 2002. J Biol Chem. 277: 20763-73. PMID: 11901144
7. Genetic Mechanisms of ADPKD.  |  Kim, DY. and Park, JH. 2016. Adv Exp Med Biol. 933: 13-22. PMID: 27730431
8. Polycystin-1 regulates cardiomyocyte mitophagy.  |  Ramírez-Sagredo, A., et al. 2021. FASEB J. 35: e21796. PMID: 34324238
9. The C-terminal tail of polycystin-1 suppresses cystic disease in a mitochondrial enzyme-dependent fashion.  |  Onuchic, L., et al. 2023. Nat Commun. 14: 1790. PMID: 36997516
10. Mechanosensitive protein polycystin-1 promotes periosteal stem/progenitor cells osteochondral differentiation in fracture healing.  |  Liu, R., et al. 2024. Theranostics. 14: 2544-2559. PMID: 38646641
11. Mechanical protein polycystin-1 directly regulates osteoclastogenesis and bone resorption.  |  Huang, M., et al. 2024. Sci Bull (Beijing). 69: 1964-1979. PMID: 38760248
12. The polycystic kidney disease 1 gene product mediates protein kinase C alpha-dependent and c-Jun N-terminal kinase-dependent activation of the transcription factor AP-1.  |  Arnould, T., et al. 1998. J Biol Chem. 273: 6013-8. PMID: 9497315

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Polycystin-1 Antibody (7E12) has 63 citations in a variety of scientific publications.

Primary antibodies like Anti-Polycystin-1 Antibody (7E12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Polycystin-1 Antibody (7E12) Specifications

Alternate Product Name: Polycystin-1

Product Clone Name: 7E12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 24-180

Epitope Target: Polycystin-1

Epitope Species: human

Product Reactivity/Detection: Polycystin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 63 citations in various scientific publications

Available Conjugates/Forms for Polycystin-1 Antibody (7E12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin