Polycystin-1 Antibody (7E12) recommended for detection of Polycystin-1 of mouse, rat and human origin by WB, IP, IF and ELISA

Polycystin-1 Antibody (7E12): sc-130554

Polycystin-1 Antibody (7E12) is rated 4.8 out of 5 by 6.
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Datasheets

    • Anti-Polycystin-1 Antibody (7E12) is a mouse monoclonal IgG1 (kappa light chain) Polycystin-1 antibody provided at 200 µg/ml
    • raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin
    • Anti-Polycystin-1 Antibody (7E12) is recommended for detection of Polycystin-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Polycystin-1 Antibody (7E12) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Polycystin-1 (7E12): sc-130554.

What's the gel percentage used for this polycystin-1 antibody in WB? Also, is there any suitable reference protein or markers?

Asked by: yybio
Thank you for your question. We used a 4-6% gel for this very large protein. These will be very fragile, so handle carefully. Please contact our Technical Service Department for suggestions of suitable high molecular weight proteins that could be used as a reference or molecular weight marker.
Answered by: Technical Support
Date published: 2019-08-17

Can you provide with a FACS protocol for this antibody (polycystin-1, 7E12) on cell lines?

Asked by: Curious2
Thank you for your question. We do not have a specific FACS protocol for use with this antibody. Please check our general protocols online, or contact our Technical Support for more information.
Answered by: Technical Support
Date published: 2019-04-13

I recently ordered this mAb conjugated to PE. I'm attempting to use this mAb to quantitate a cell surface antigen - to do that I need to know the ratio of PE molecules per mAb. I.E., do you know if there is one PE bound to each mAb, or two, etc?

Asked by: Jeff R
Thank you for your question. For PE conjugated antibodies, the ratio is 1:1, so there is 1 PE per antibody molecule.
Answered by: Tech Service
Date published: 2019-03-25

Which part does this antibody (polycystin-1, 7E12) recognize?

Asked by: yybio
Thank you for your question. This monoclonal antibody was raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin. We will update our product description for future reference.
Answered by: Technical Support
Date published: 2018-12-10

Is it possible to use the AC conjugated Polycystin-1 Antibody (7E12): sc-130554 AC in both Western Blot and IP?

Asked by: jenni
Thank you for your inquiry. We do not recommend the use of the AC conjugated form in Western blot. The AC conjugated form of the antibody is specifically used for IP. The unconjugated form of the antibody is the best choice when working in multiple applications.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from Good for your Western blot! An excellent detection reagent for your WB. If you need to visualize polycystin-1 in human samples, this antibody can do it. Provides a nice clear band and has been cited over ten times.
Date published: 2017-01-16
Rated 4 out of 5 by from Strong western Very nice western blot data; strong signal with low background
Date published: 2017-01-06
Rated 5 out of 5 by from Used this antibody in WB and got good results Used this antibody in WB and got good results.
Date published: 2015-08-17
Rated 5 out of 5 by from Successful application of this antibody in Successful application of this antibody in WB using human PDL fibroblasts -SCBT Publication Review
Date published: 2015-04-19
Rated 5 out of 5 by from WB publishable data with mIMCD cells WB publishable data with mIMCD cells - SCBT Publication Review
Date published: 2015-03-30
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for WB on human HEK293 cells - SCBT Publication Review
Date published: 2015-03-18
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