POLR3B Antibody (G-6): sc-515362
- POLR3B Antibody (G-6) is a mouse monoclonal IgG1 κ POLR3B antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 92-111 near the N-terminus of POLR3B of human origin
- POLR3B Antibody (G-6) is recommended for detection of POLR3B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-POLR3B Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for POLR3B Antibody (G-6) for WB applications. This reagent is now offered in a bundle with POLR3B Antibody (G-6) (see ordering information below).
POLR3B Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects POLR3B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POLR3B (G-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLR3B protein, also known as polymerase (RNA) III (DNA directed) polypeptide B, is a crucial component of RNA polymerase III complex, which is responsible for synthesizing small RNAs, such as transfer RNA and ribosomal RNA, essential for protein synthesis and cellular function. This 1,133 amino acid protein is located in nucleus, where POLR3B plays a vital role in transcription process, converting DNA into RNA using ribonucleoside triphosphates as substrates. Proper functioning of POLR3B is critical, as defects in RNA polymerase III activity can lead to various diseases, including neurodegenerative disorders and certain cancers. POLR3B gene is situated on human chromosome 12, a region associated with several genetic disorders, highlighting POLR3B′s importance in both normal cellular processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POLR3B Antibody (G-6) References:
- Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. | Hu, P., et al. 2002. Mol Cell Biol. 22: 8044-55. PMID: 12391170
- The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC. | Jang, KL., et al. 1992. J Acquir Immune Defic Syndr (1988). 5: 1142-7. PMID: 1403646
- Genotype/phenotype analysis in a patient with pure and complete trisomy 12p. | Zumkeller, W., et al. 2004. Am J Med Genet A. 129A: 261-4. PMID: 15326625
- The natural history of trisomy 12p. | Segel, R., et al. 2006. Am J Med Genet A. 140: 695-703. PMID: 16502429
- Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. | Shen, E., et al. 2009. Exp Cell Res. 315: 1100-16. PMID: 19331820
- Clinical spectrum of 4H leukodystrophy caused by POLR3A and POLR3B mutations. | Wolf, NI., et al. 2014. Neurology. 83: 1898-905. PMID: 25339210
- De novo variants in POLR3B cause ataxia, spasticity, and demyelinating neuropathy. | Djordjevic, D., et al. 2021. Am J Hum Genet. 108: 186-193. PMID: 33417887
- Identification of POLR3B biallelic mutations-associated hypomyelinating leukodystrophy-8 in two siblings. | Yang, F., et al. 2023. Clin Genet. 103: 596-602. PMID: 36650939
- Polr3b heterozygosity in mice induces both beneficial and deleterious effects on health during ageing with no effect on lifespan. | Borland, G., et al. 2024. Aging Cell. 23: e14141. PMID: 38465473
- POLR3B is associated with a developmental and epileptic encephalopathy with myoclonic-atonic seizures and ataxia. | Symonds, JD., et al. 2024. Epilepsia. 65: 3303-3323. PMID: 39348199
- Cytogenetic and molecular analysis in trisomy 12p. | Allen, TL., et al. 1996. Am J Med Genet. 63: 250-6. PMID: 8723118
- Low basal transcripts of the COL2A1 collagen gene from lymphoblasts show alternative splicing of exon 12 in the Kniest form of spondyloepiphyseal dysplasia. | Yang, W. and Cole, WG. 1998. Hum Mutat. Suppl 1: S1-2. PMID: 9452022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POLR3B Antibody (G-6) | sc-515362 | 200 µg/ml | $322.00 | |||
POLR3B Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-526434 | 200 µg Ab; 10 µg BP | $361.00 | |||
POLR3B Antibody (G-6) AC | sc-515362 AC | 500 µg/ml, 25% agarose | $424.00 | |||
POLR3B Antibody (G-6) HRP | sc-515362 HRP | 200 µg/ml | $322.00 | |||
POLR3B Antibody (G-6) FITC | sc-515362 FITC | 200 µg/ml | $336.00 | |||
POLR3B Antibody (G-6) PE | sc-515362 PE | 200 µg/ml | $349.00 | |||
POLR3B Antibody (G-6) Alexa Fluor® 488 | sc-515362 AF488 | 200 µg/ml | $364.00 | |||
POLR3B Antibody (G-6) Alexa Fluor® 546 | sc-515362 AF546 | 200 µg/ml | $364.00 | |||
POLR3B Antibody (G-6) Alexa Fluor® 594 | sc-515362 AF594 | 200 µg/ml | $364.00 | |||
POLR3B Antibody (G-6) Alexa Fluor® 647 | sc-515362 AF647 | 200 µg/ml | $364.00 | |||
POLR3B Antibody (G-6) Alexa Fluor® 680 | sc-515362 AF680 | 200 µg/ml | $364.00 | |||
POLR3B Antibody (G-6) Alexa Fluor® 790 | sc-515362 AF790 | 200 µg/ml | $364.00 | |||
POLR3B (G-6) Neutralizing Peptide | sc-515362 P | 100 µg/0.5 ml | $69.00 |