POLR2E Antibody (B-5): sc-390979

POLR2E Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects POLR2E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-POLR2E antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLR2E protein, also known as polymerase (RNA) II (DNA directed) polypeptide E, RPB5, XAP4, RPABC1, hRPB25, or hsRPB5, is a crucial 210 amino acid nuclear protein that plays a significant role in the transcription process by forming part of the RNA polymerase II complex. This complex is essential for synthesizing messenger RNA (mRNA) and ribosomal RNA precursors, as well as various functional non-coding RNAs and small RNAs, including 5S rRNA and transfer RNAs. POLR2E is particularly important because POLR2E interacts with TFIIF RAP 30, facilitating the association between RNA polymerase II and TFIIF, which is vital for the transcription initiation process. Understanding POLR2E function and interactions is critical for elucidating the mechanisms of gene expression regulation and the overall transcriptional landscape in eukaryotic cells.

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POLR2E Antibody (B-5) References:

1. Direct interaction between the subunit RAP30 of transcription factor IIF (TFIIF) and RNA polymerase subunit 5, which contributes to the association between TFIIF and RNA polymerase II.  |  Wei, W., et al. 2001. J Biol Chem. 276: 12266-73. PMID: 11278533
2. Subcellular localization of RPB5-mediating protein and its putative functional partner.  |  Delgermaa, L., et al. 2004. Mol Cell Biol. 24: 8556-66. PMID: 15367675
3. Mutational analysis of human RNA polymerase II subunit 5 (RPB5): the residues critical for interactions with TFIIF subunit RAP30 and hepatitis B virus X protein.  |  Le, TT., et al. 2005. J Biochem. 138: 215-24. PMID: 16169872
4. RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor.  |  Panov, KI., et al. 2006. Mol Cell Biol. 26: 5436-48. PMID: 16809778
5. Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.  |  Zaros, C., et al. 2007. Nucleic Acids Res. 35: 634-47. PMID: 17179178
6. High-resolution mapping of the protein interaction network for the human transcription machinery and affinity purification of RNA polymerase II-associated complexes.  |  Cloutier, P., et al. 2009. Methods. 48: 381-6. PMID: 19450687
7. Screening for genetic abnormalities involved in ovarian carcinogenesis using retroviral expression libraries.  |  Wada, T., et al. 2009. Int J Oncol. 35: 973-6. PMID: 19787249
8. Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitis B virus X protein and may play a role in X transactivation.  |  Cheong, JH., et al. 1995. EMBO J. 14: 143-50. PMID: 7828586
9. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes.  |  Kershnar, E., et al. 1998. J Biol Chem. 273: 34444-53. PMID: 9852112

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-POLR2E Antibody (B-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-POLR2E Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

POLR2E Antibody (B-5) Specifications

Alternate Product Name: POLR2E

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 7-210

Epitope Target: POLR2E

Epitope Species: human

Product Reactivity/Detection: POLR2E

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for POLR2E Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin