Pol II RPB6 Antibody (E-8): sc-271309
- Pol II RPB6 Antibody (E-8) is a mouse monoclonal IgG1 κ Pol II RPB6 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-127 representing full length Pol II RPB6 of human origin
- Pol II RPB6 Antibody (E-8) is recommended for detection of Pol II RPB6 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Pol II RPB6 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Pol II RPB6 Antibody (E-8) for WB applications. These reagents are now offered in bundles with Pol II RPB6 Antibody (E-8) (see ordering information below).
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Pol II RPB6 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pol II RPB6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pol II RPB6 antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pol II RPB6 protein is a critical component of RNA polymerase II, which is essential for the transcription of protein-coding genes. Pol II RPB6 plays a vital role in the assembly of the transcription machinery at the promoter, facilitating the recruitment of transcription factors and chromatin remodeling complexes necessary for gene expression. Pol II RPB6 subunit is integral to the stability and functionality of the RNA polymerase II complex, as Pol II RPB6 helps to coordinate the interaction between the polymerase and various transcription factors, ensuring accurate transcription initiation. Understanding Pol II RPB6 function is crucial for elucidating the mechanisms of gene regulation and the overall process of transcription, which is fundamental to cellular function and development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pol II RPB6 Antibody (E-8) References:
- Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms. | Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
- Structure and function of RNA polymerase II. | Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
- Mechanism of RNA polymerase I transcription. | Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
- The RNA polymerase II transcription cycle: cycling through chromatin. | Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
- RNA polymerase III transcription and cancer. | White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II. | Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
- Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity. | Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
- Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein. | Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
- Downstream DNA selectively affects a paused conformation of human RNA polymerase II. | Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pol II RPB6 Antibody (E-8) | sc-271309 | 200 µg/ml | $322.00 | |||
Pol II RPB6 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-527230 | 200 µg Ab; 10 µg BP | $361.00 | |||
Pol II RPB6 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-532603 | 200 µg Ab; 20 µg BP | $361.00 | |||
Pol II RPB6 Antibody (E-8) AC | sc-271309 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Pol II RPB6 Antibody (E-8) HRP | sc-271309 HRP | 200 µg/ml | $322.00 | |||
Pol II RPB6 Antibody (E-8) FITC | sc-271309 FITC | 200 µg/ml | $336.00 | |||
Pol II RPB6 Antibody (E-8) PE | sc-271309 PE | 200 µg/ml | $349.00 | |||
Pol II RPB6 Antibody (E-8) Alexa Fluor® 488 | sc-271309 AF488 | 200 µg/ml | $364.00 | |||
Pol II RPB6 Antibody (E-8) Alexa Fluor® 546 | sc-271309 AF546 | 200 µg/ml | $364.00 | |||
Pol II RPB6 Antibody (E-8) Alexa Fluor® 594 | sc-271309 AF594 | 200 µg/ml | $364.00 | |||
Pol II RPB6 Antibody (E-8) Alexa Fluor® 647 | sc-271309 AF647 | 200 µg/ml | $364.00 | |||
Pol II RPB6 Antibody (E-8) Alexa Fluor® 680 | sc-271309 AF680 | 200 µg/ml | $364.00 | |||
Pol II RPB6 Antibody (E-8) Alexa Fluor® 790 | sc-271309 AF790 | 200 µg/ml | $364.00 |