Pol I/II/III RPB6 Antibody (B6-1): sc-21751

Pol I/II/III RPB6 Antibody (B6-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Pol I/II/III RPB6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pol I/II/III RPB6 antibody (B6-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Pol I/II/III RPB6 protein plays a crucial role in the assembly and function of the three distinct classes of RNA polymerases found in eukaryotic cells, namely RNA polymerase I, II, and III, which are responsible for synthesizing different types of RNA. Specifically, the RPB6 subunit is essential for the stability and activity of these polymerases, as RPB6 is a shared component that contributes to the formation of the core enzyme complex. This shared nature underscores the importance of RPB6 in regulating gene expression and maintaining cellular function, as any disruption in RPB6 activity can lead to significant consequences in RNA synthesis and, consequently, protein production. Pol I/II/III RPB6 monoclonal antibody (B6-1) effectively detects this critical protein across multiple species, making B6-1 an invaluable tool for researchers studying transcriptional regulation and RNA polymerase function in various biological contexts.

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Pol I/II/III RPB6 Antibody (B6-1) References:

1. Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
2. Structure and function of RNA polymerase II.  |  Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
3. Mechanism of RNA polymerase I transcription.  |  Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
4. The RNA polymerase II transcription cycle: cycling through chromatin.  |  Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
5. RNA polymerase III transcription and cancer.  |  White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
6. BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
7. Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity.  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
8. Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein.  |  Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
9. Downstream DNA selectively affects a paused conformation of human RNA polymerase II.  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pol I/II/III RPB6 Antibody (B6-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Pol I/II/III RPB6 Antibody (B6-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pol I/II/III RPB6 Antibody (B6-1) Specifications

Alternate Product Name: Pol I/II/III RPB6

Product Clone Name: B6-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Pol I/II/III RPB6

Epitope Species: human

Product Reactivity/Detection: Pol I/II/III RPB6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Pol I/II/III RPB6 Antibody (B6-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Pol II RPB6 antibody is Pol II RPB6 Antibody (E-8): sc-271309.