Pol II Antibody (CTD4H8): sc-47701

Pol II Antibody (CTD4H8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Pol II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pol II antibody (CTD4H8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNA polymerase II (Pol II) plays a crucial role in the transcription of protein-coding genes, and Pol II function is vital for gene expression regulation. The enzyme is composed of twelve subunits, with the largest subunit, RPB1 (also known as RPB205), being a 1,840 amino acid protein that features a C2H2-type zinc finger and a C-terminal domain rich in heptapeptide repeats. The structural integrity of RPB1 is essential, as RPB1 not only conveys the catalytic activity of Pol II but also contributes to the formation of the DNA-binding domain, which is critical for the transcription process. The interaction between RPB1 and the other subunits ensures that the transcription machinery is effectively recruited to target promoters, facilitating the initiation of mRNA synthesis. Disruption in Pol II function, particularly through alterations in the largest subunit, can lead to significant impairments in gene expression, underscoring RPB1′s importance in cellular function and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pol II Antibody (CTD4H8) has 112 citations in a variety of scientific publications.

Primary antibodies like Anti-Pol II Antibody (CTD4H8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pol II Antibody (CTD4H8) Specifications

Alternate Product Name: RNA Polymerase II

Product Clone Name: CTD4H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Pol II

Epitope Species: human

Product Reactivity/Detection: hypo- and hyper-phosphorylated Pol II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 112 citations in various scientific publications

Available Conjugates/Forms for Pol II Antibody (CTD4H8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Pol II antibody is Pol II Antibody (8WG16): sc-56767.