Pol II Antibody (3WG2): sc-21750
- Pol II Antibody (3WG2) is a mouse monoclonal IgM κ, cited in 7 publications, provided at 200 µg/ml
- raised against conserved heptapeptide repeat on the largest subunit of RNA polymerase II
- recommended for detection of Pol II of mouse, rat and human origin by WB and IP
- See Pol II (CTD4H8): sc-47701 for Pol II antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Pol II Antibody (3WG2) for WB applications. This reagent is now offered in a bundle with Pol II Antibody (3WG2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SEE ALSO...
Pol II Antibody (3WG2) is a mouse monoclonal IgM antibody that detects RNA polymerase II (Pol II) in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Pol II is a crucial enzyme composed of twelve subunits, responsible for the transcription of protein-coding genes, which is a fundamental process for gene expression and cellular function. RPB1 or RPB205, the largest subunit of Pol II, is a significant 1,840 amino acid protein that features a C2H2-type zinc finger and a C-terminal domain rich in heptapeptide repeats. This large subunit not only conveys the catalytic activity of Pol II but also plays a vital role in forming the DNA-binding domain, which is essential for the accurate transcription of the DNA template. Without proper function of RPB1, mRNA synthesis and transcription elongation are severely compromised, leading to potential disruptions in cellular processes and gene regulation. Anti-Pol II antibody (3WG2) serves as an invaluable tool for researchers studying the mechanisms of transcription and the regulation of gene expression in various biological contexts.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pol II Antibody (3WG2) | sc-21750 | 200 µg/ml | $322.00 | |||
Pol II Antibody (3WG2): m-IgGκ BP-HRP Bundle | sc-550731 | 200 µg Ab; 40 µg BP | $361.00 |