Pol II Antibody (1.BB.61): sc-71917

Pol II Antibody (1.BB.61) is a mouse monoclonal IgG2a antibody that detects RNA polymerase II (Pol II) across various species including mouse, rat, human, Xenopus, C. elegans, yeast, wheat germ, and bovine, and is applicable for western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Pol II is a crucial enzyme composed of twelve subunits, responsible for the transcription of protein-coding genes, and proper function is vital for gene expression regulation. The largest subunit of Pol II, known as RPB1 or RPB205, is a 1,840 amino acid protein that features a C2H2-type zinc finger and a C-terminal domain rich in heptapeptide repeats. This subunit not only conveys the catalytic activity of Pol II but also plays a significant role in forming the DNA-binding domain, which is essential for the transcription of the DNA template. The integrity of the large subunit is critical; without this component, mRNA synthesis and transcription elongation cannot proceed, highlighting the importance of Pol II in cellular function and gene regulation. RNA polymerase II (Pol II) antibody (1.BB.61) is an invaluable tool for researchers studying transcription mechanisms and gene expression across a variety of model organisms.

The preferred Pol II antibody is Pol II Antibody (8WG16): sc-56767.