Date published: 2026-1-11

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Pol I/II/III RPB8 Antibody (SP-75): sc-134422

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Datasheets
  • Pol I/II/III RPB8 Antibody (SP-75) is a mouse monoclonal IgG1 κ provided at 100 µg/ml
  • raised against recombinant Pol I/II/III RPB8 protein of human origin
  • recommended for detection of RNA polymerase I/II/III subunit RPB8 of mouse, rat and human origin by WB, IP and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Pol I/II/III RPB8 Antibody (SP-75). This work is in progress.

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    Pol I/II/III RPB8 Antibody (SP-75) is a mouse monoclonal IgG1 antibody that detects RPB8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-RPB8 antibody (SP-75) plays a crucial role in the study of RNA polymerases, as RPB8 is a vital subunit shared among all three classes of RNA polymerases—Pol I, Pol II, and Pol III—responsible for synthesizing different types of RNA. RPB8 is located in the nucleus, where RPB8 contributes to the assembly and stability of RNA polymerase complexes, which are essential for transcription of ribosomal RNA, messenger RNA, and transfer RNA. Understanding RPB8 localization is important because this highlights RPB8′s role in regulating gene expression and maintaining cellular function. Proper functioning of RNA polymerases is critical for cellular processes, including growth, differentiation, and response to environmental signals, making RPB8 a significant target for research in molecular biology and genetics. With RPB8′s ability to interact with various other subunits and proteins involved in transcription, RPB8 monoclonal antibody (SP-75) is an invaluable tool for researchers aiming to explore the complexities of gene regulation and RNA synthesis.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Pol I/II/III RPB8 Antibody (SP-75)

    sc-134422
    100 µg/ml
    $339.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with Pol I/II/III RPB8 (SP-75): sc-134422 antibody?

    Asked by: jenniferc
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 5 out of 5 by from WB: Single strong band in HeLa whole cell lysateWB: Single strong band in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-02-04
    Rated 5 out of 5 by from Worked great for WesternWorked great for Western, Pol I/II/III RPB8 expression in Hep G2 whole cell lysate. -SCBT QC
    Date published: 2013-11-30
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    Pol I/II/III RPB8 Antibody (SP-75) is rated 5.0 out of 5 by 2.
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