Pol I/II/III RPB8 Antibody (4WG2): sc-57982

Pol I/II/III RPB8 Antibody (4WG2) is a mouse monoclonal IgG2a antibody that detects Pol I RPB8, Pol II RPB8, and Pol III RPB8 proteins of yeast origin by western blotting (WB). Anti-Pol I/II/III RPB8 antibody (4WG2) is available in a non-conjugated form, providing researchers with a versatile tool for studying RNA polymerase function. RPB8 plays a crucial role in the assembly and stability of RNA polymerase complexes, which are essential for the transcription of various RNA types. RPB8 functions as a component of all three eukaryotic RNA polymerases, Pol I, II, and III, and maintains vital roles in proper enzyme function. RPB8 interaction with other subunits, such as RPB6, ensures structural integrity of polymerases, allowing efficient transcription of ribosomal RNA, messenger RNA, and transfer RNA. Understanding RPB8 function and interactions helps elucidate mechanisms of gene expression and regulation in eukaryotic cells, making anti-Pol I/II/III RPB8 antibody (4WG2) an invaluable resource for researchers investigating transcriptional processes.

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Pol I/II/III RPB8 Antibody (4WG2) References:

1. Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
2. Structure and function of RNA polymerase II.  |  Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
3. Mechanism of RNA polymerase I transcription.  |  Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
4. The RNA polymerase II transcription cycle: cycling through chromatin.  |  Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
5. RNA polymerase III transcription and cancer.  |  White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
6. BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
7. Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity.  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
8. Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein.  |  Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
9. Downstream DNA selectively affects a paused conformation of human RNA polymerase II.  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834

The preferred Pol I/II/III RPB8 antibody is Pol I/II/III RPB8 Antibody (B-2): sc-398512.