PME-1 Antibody (F-11): sc-137161

PME-1 Antibody (F-11) is a mouse monoclonal IgG1 antibody that detects protein phosphatase methylesterase-1 (PME-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PME-1 plays a crucial role in regulating protein phosphatase 2A (PP2A) activity, a key enzyme involved in cell growth inhibition and cell cycle regulation. By catalyzing PP2A demethylation and inactivation, PME-1 influences various cellular processes, including proliferation and apoptosis, making PME-1 essential for maintaining cellular homeostasis. PME-1 is primarily located in the cytoplasm, where PME-1 interacts with PP2A and other regulatory proteins, underscoring PME-1′s importance in modulating signaling pathways that govern cell fate decisions. PME-1 is highly conserved across species, from yeast to humans, and features a catalytic triad similar to that found in lipases, which is critical for enzymatic function. PME-1′s ability to demethylate PP2A catalytic subunit in vitro, along with okadaic acid inhibition of this reaction, highlights PME-1′s potential as a therapeutic target in diseases characterized by dysregulated phosphatase activity. Anti-PME-1 antibody (F-11) is an invaluable tool for researchers studying cell signaling mechanisms and phosphatase roles in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PME-1 Antibody (F-11) References:

1. Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice.  |  Ortega-Gutiérrez, S., et al. 2008. PLoS One. 3: e2486. PMID: 18596935
2. Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.  |  Sontag, JM., et al. 2010. J Neurochem. 115: 1455-65. PMID: 21044074
3. Interaction between salt-inducible kinase 2 and protein phosphatase 2A regulates the activity of calcium/calmodulin-dependent protein kinase I and protein phosphatase methylesterase-1.  |  Lee, CW., et al. 2014. J Biol Chem. 289: 21108-19. PMID: 24841198
4. Carnosic acid stimulates glucose uptake in skeletal muscle cells via a PME-1/PP2A/PKB signalling axis.  |  Lipina, C. and Hundal, HS. 2014. Cell Signal. 26: 2343-9. PMID: 25038454
5. Protein phosphatase methylesterase-1 (PME-1) expression predicts a favorable clinical outcome in colorectal cancer.  |  Kaur, A., et al. 2015. Cancer Med. 4: 1798-808. PMID: 26377365
6. Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.  |  Yabe, R., et al. 2015. PLoS One. 10: e0145226. PMID: 26678046
7. Regulation of protein phosphatase 2A (PP2A) tumor suppressor function by PME-1.  |  Kaur, A. and Westermarck, J. 2016. Biochem Soc Trans. 44: 1683-1693. PMID: 27913678
8. A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation.  |  Yabe, R., et al. 2018. FEBS Open Bio. 8: 1486-1496. PMID: 30186749
9. Pseudomonas aeruginosa with NDM-1, DIM-1 and PME-1 β-lactamases, and RmtD3 16S rRNA methylase, encoded by new genomic islands.  |  Urbanowicz, P., et al. 2019. J Antimicrob Chemother. 74: 3117-3119. PMID: 31211367
10. PP2A methylesterase PME-1 suppresses anoikis and is associated with therapy relapse of PTEN-deficient prostate cancers.  |  Aakula, A., et al. 2023. Mol Oncol. 17: 1007-1023. PMID: 36461911

The preferred PME-1 antibody is PME-1 Antibody (B-12): sc-25278.