PMCA3 Antibody (G-6): sc-390148

PMCA3 Antibody (G-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects PMCA3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PMCA3 Antibody (G-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PMCA3 plays a crucial role in regulating intracellular calcium levels, which is vital for numerous cellular processes, including muscle contraction, neurotransmitter release, and cell signaling. PMCA3 is predominantly expressed in brain and skeletal muscle, where PMCA3 helps maintain calcium homeostasis by exporting calcium ions out of cells, thus preventing calcium overload that can lead to cellular dysfunction or apoptosis. Precise regulation of PMCA3 expression is essential for proper functioning of excitable tissues, as fluctuations in calcium levels can significantly impact neuronal excitability and muscle contraction. Understanding PMCA3 dynamics and isoforms is critical for elucidating mechanisms underlying various physiological and pathological conditions, making anti-PMCA3 antibody (G-6) an invaluable tool for researchers studying calcium signaling pathways in these tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PMCA3 Antibody (G-6) References:

1. Plasma membrane calcium ATPase isoforms in astrocytes.  |  Fresu, L., et al. 1999. Glia. 28: 150-5. PMID: 10533058
2. Plasma membrane calcium ATPases as critical regulators of calcium homeostasis during neuronal cell function.  |  Garcia, ML. and Strehler, EE. 1999. Front Biosci. 4: D869-82. PMID: 10577388
3. Role of alternative splicing in generating isoform diversity among plasma membrane calcium pumps.  |  Strehler, EE. and Zacharias, DA. 2001. Physiol Rev. 81: 21-50. PMID: 11152753
4. Delayed activation of the plasma membrane calcium pump by a sudden increase in Ca2+: fast pumps reside in fast cells.  |  Caride, AJ., et al. 2001. Cell Calcium. 30: 49-57. PMID: 11396987
5. Localization of two genes encoding plasma membrane Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and 12q21-23.  |  Olson, S., et al. 1991. Genomics. 9: 629-41. PMID: 1674727
6. Inhibitory interaction of the 14-3-3 proteins with ubiquitous (PMCA1) and tissue-specific (PMCA3) isoforms of the plasma membrane Ca2+ pump.  |  Linde, CI., et al. 2008. Cell Calcium. 43: 550-61. PMID: 18029012
7. GABA-shunt enzymes activity in GH3 cells with reduced level of PMCA2 or PMCA3 isoform.  |  Kowalski, A., et al. 2011. Biochem Biophys Res Commun. 411: 815-20. PMID: 21798237
8. Gene expression pattern in PC12 cells with reduced PMCA2 or PMCA3 isoform: selective up-regulation of calmodulin and neuromodulin.  |  Boczek, T., et al. 2012. Mol Cell Biochem. 360: 89-102. PMID: 21912933
9. Downregulation of PMCA2 or PMCA3 reorganizes Ca(2+) handling systems in differentiating PC12 cells.  |  Boczek, T., et al. 2012. Cell Calcium. 52: 433-44. PMID: 22921123
10. A novel PMCA3 mutation in an ataxic patient with hypomorphic phosphomannomutase 2 (PMM2) heterozygote mutations: Biochemical characterization of the pump defect.  |  Vicario, M., et al. 2017. Biochim Biophys Acta Mol Basis Dis. 1863: 3303-3312. PMID: 28807751
11. The ataxia-linked E1081Q mutation affects the sub-plasma membrane Ca2+-microdomains by tuning PMCA3 activity.  |  Vallese, F., et al. 2022. Cell Death Dis. 13: 855. PMID: 36207321
12. Localization of the plasma membrane Ca(2+)-ATPase isoform PMCA3 in rat cerebellum, choroid plexus and hippocampus.  |  Eakin, TJ., et al. 1995. Brain Res Mol Brain Res. 29: 71-80. PMID: 7770003

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PMCA3 Antibody (G-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PMCA3 Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PMCA3 Antibody (G-6) Specifications

Alternate Product Name: PMCA3

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1133-1167

Epitope Target: PMCA3

Epitope Species: human

Product Reactivity/Detection: all isoforms of PMCA3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PMCA3 Antibody (G-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PMCA antibody is PMCA Antibody (F-3): sc-271917.