PMCA Antibody (F-3): sc-271917

PMCA Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PMCA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PMCA (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Plasma membrane-type Ca2+-ATPases (PMCAs) play a crucial role in maintaining calcium homeostasis by mediating the export of bivalent calcium ions from eukaryotic cells, which is essential for numerous cellular processes, including muscle contraction, neurotransmitter release, and cell signaling. PMCA protein is located in the plasma membrane, where active pumping of calcium ions out of cells prevents toxic calcium overload and ensures proper cellular function. PMCA expression regulation is tightly controlled and varies across different tissues and developmental stages, allowing cells to adapt to specific physiological needs. Understanding PMCA function dynamics and regulation is vital for elucidating roles in health and disease, particularly in conditions associated with calcium dysregulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PMCA Antibody (F-3) References:

1. Plasma membrane calcium ATPase isoforms in astrocytes.  |  Fresu, L., et al. 1999. Glia. 28: 150-5. PMID: 10533058
2. Role of alternative splicing in generating isoform diversity among plasma membrane calcium pumps.  |  Strehler, EE. and Zacharias, DA. 2001. Physiol Rev. 81: 21-50. PMID: 11152753
3. Delayed activation of the plasma membrane calcium pump by a sudden increase in Ca2+: fast pumps reside in fast cells.  |  Caride, AJ., et al. 2001. Cell Calcium. 30: 49-57. PMID: 11396987
4. Plasma membrane Ca(2+)-ATPase (PMCA) translocates to filopodia during platelet activation.  |  Dean, WL. and Whiteheart, SW. 2004. Thromb Haemost. 91: 325-33. PMID: 14961161
5. Localization of two genes encoding plasma membrane Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and 12q21-23.  |  Olson, S., et al. 1991. Genomics. 9: 629-41. PMID: 1674727
6. Platelet PMCA- and SERCA-type Ca2+ -ATPase expression in diabetes: a novel signature of abnormal megakaryocytopoiesis.  |  Chaabane, C., et al. 2007. J Thromb Haemost. 5: 2127-35. PMID: 17883705
7. STIM1 is required for attenuation of PMCA-mediated Ca2+ clearance during T-cell activation.  |  Ritchie, MF., et al. 2012. EMBO J. 31: 1123-33. PMID: 22246182
8. Hyperactivation of the human plasma membrane Ca2+ pump PMCA h4xb by mutation of Glu99 to Lys.  |  Mazzitelli, LR. and Adamo, HP. 2014. J Biol Chem. 289: 10761-10768. PMID: 24584935
9. Molecular cloning of a third isoform of the calmodulin-sensitive plasma membrane Ca2+-transporting ATPase that is expressed predominantly in brain and skeletal muscle.  |  Greeb, J. and Shull, GE. 1989. J Biol Chem. 264: 18569-76. PMID: 2530223
10. Detecting Alpha Synuclein Seeding Activity in Formaldehyde-Fixed MSA Patient Tissue by PMCA.  |  Becker, K., et al. 2018. Mol Neurobiol. 55: 8728-8737. PMID: 29589283
11. The Ca2+ export pump PMCA clears near-membrane Ca2+ to facilitate store-operated Ca2+ entry and NFAT activation.  |  Go, CK., et al. 2019. Sci Signal. 12: PMID: 31594854
12. PMCA to demonstrate the efficacy of prion inactivation methods on reusable medical devices: a relevant alternative to animal bioassays.  |  Igel, A., et al. 2024. J Hosp Infect. 154: 60-63. PMID: 39357541

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PMCA Antibody (F-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PMCA Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PMCA Antibody (F-3) Specifications

Alternate Product Name: PMCA

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 481-780

Epitope Target: PMCA

Epitope Species: human

Product Reactivity/Detection: PMCA1, PMCA2, PMCA3 and PMCA4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PMCA Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin