PLC δ1 Antibody (D-10): sc-374329

PLC δ1 Antibody (D-10) is a mouse monoclonal IgG1 antibody that detects phosphoinositide-specific phospholipase C δ1 (PLC δ1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PLC δ1 plays a pivotal role in calcium signaling, acting as a calcium signal amplifier that is activated by atypical GTP-binding proteins, thereby facilitating critical receptor-mediated signal transduction pathways. PLC δ1 is predominantly expressed in the brain, heart, lung, and testis, and abnormal PLC δ1 accumulation has been observed in the brains of individuals with Alzheimer′s disease, indicating a potential involvement in the disease′s pathology. PLC δ1 presence in these tissues underscores its importance in various physiological processes, including neuronal signaling and cardiovascular function. Additionally, PLC δ1 interacts with several other proteins, enhancing its role in cellular signaling networks. PLC δ1′s ability to generate second messengers inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate is crucial for propagating signals that regulate diverse cellular responses, making PLC δ1 a significant target for research in neurodegenerative diseases and other conditions related to calcium signaling dysregulation. Anti-PLC δ1 antibody (D-10) is an essential tool for researchers aiming to explore the intricate mechanisms of signal transduction and the pathological implications of PLC δ1 in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PLC δ1 Antibody (D-10) References:

1. Phospholipase C-delta1 is activated by capacitative calcium entry that follows phospholipase C-beta activation upon bradykinin stimulation.  |  Kim, YH., et al. 1999. J Biol Chem. 274: 26127-34. PMID: 10473563
2. Phospholipase C-delta1 rescues intracellular Ca2+ overload in ischemic heart and hypoxic neonatal cardiomyocytes.  |  Hwang, KC., et al. 2004. J Steroid Biochem Mol Biol. 91: 131-8. PMID: 15276620
3. Recruitment and activation of phospholipase C (PLC)-delta1 in lipid rafts by muscarinic stimulation of PC12 cells: contribution of p122RhoGAP/DLC1, a tumor-suppressing PLCdelta1 binding protein.  |  Yamaga, M., et al. 2008. Adv Enzyme Regul. 48: 41-54. PMID: 18157946
4. Phospholipase C-delta1 modulates sustained contraction of rat mesenteric small arteries in response to noradrenaline, but not endothelin-1.  |  Clarke, CJ., et al. 2008. Am J Physiol Heart Circ Physiol. 295: H826-34. PMID: 18567701
5. Ral GTPase interacts with the N-terminal in addition to the C-terminal region of PLC-delta1.  |  Grujic, O. and Bhullar, RP. 2009. Biochem Biophys Res Commun. 383: 401-5. PMID: 19379713
6. PLCδ1 protein rescues ischemia-reperfused heart by the regulation of calcium homeostasis.  |  Lim, S., et al. 2014. Mol Ther. 22: 1110-1121. PMID: 24637455
7. The low PLC-δ1 expression in cystic fibrosis bronchial epithelial cells induces upregulation of TRPV6 channel activity.  |  Vachel, L., et al. 2015. Cell Calcium. 57: 38-48. PMID: 25477137
8. Phospholipase C δ1 in macrophages negatively regulates TLR4-induced proinflammatory cytokine production and Fcγ receptor-mediated phagocytosis.  |  Kudo, K., et al. 2016. Adv Biol Regul. 61: 68-79. PMID: 26643908
9. Phospholipase C δ1 negatively regulates autophagy in colorectal cancer cells.  |  Shimozawa, M., et al. 2017. Biochem Biophys Res Commun. 488: 578-583. PMID: 28528980
10. The Gαh-PLCδ1 signaling axis drives metastatic progression in triple-negative breast cancer.  |  Huang, SP., et al. 2017. J Hematol Oncol. 10: 114. PMID: 28576130

The preferred PLC δ1 antibody is PLC δ1 Antibody (D-7): sc-393464.