PLC delta 1 Antibody (A-4) recommended for detection of PLC δ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PLC δ1 Antibody (A-4): sc-365812

PLC δ1 Antibody (A-4) is rated 4.7 out of 5 by 3.
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Datasheets

    • PLC δ1 Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-140 mapping at the N-terminus of PLC δ1 of human origin
    • recommended for detection of PLC δ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

Can PLC δ1 (A-4): sc-365812 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, PLC δ1 (A-4): sc-365812 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent Our old antibody from another company wasn't working properly, so we purchased this one. Worked even better than the old one.
Date published: 2017-03-29
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-02-28
Rated 4 out of 5 by from Robust band seen in WB using F9 whole cell lysate Robust band seen in WB using F9 whole cell lysate. -SCBT QC
Date published: 2015-02-23
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