PLC δ1 Antibody (A-4): sc-365812

PLC δ1 Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects phosphoinositide-specific phospholipase C δ1 (PLC δ1) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PLC δ1 plays a pivotal role in calcium signaling, acting as a calcium signal amplifier that is activated by atypical GTP-binding proteins. This activation is crucial for signaling pathways mediated by receptors such as the oxytocin receptor and the α1B-adrenoreceptor, which are essential for various physiological processes. PLC δ1 is predominantly expressed in the brain, heart, lung, and testis, and abnormal PLC δ1 accumulation has been observed in the brains of individuals with Alzheimer′s disease, indicating a potential involvement in the disease′s pathology. Additionally, PLC δ1 interacts with other phospholipase C family members, such as PLC δ3 and PLC δ4, which also contain functional domains that bind calcium and catalyze the production of second messengers diacylglycerol and inositol 1,4,5-triphosphate. The presence of PLC δ1 in various tissues underscores its importance in regulating cellular signaling and function, making PLC δ1 a significant target for research in both normal physiology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PLC δ1 Antibody (A-4) References:

1. Amyloid beta peptide (Abeta42) activates PLC-delta1 promoter through the NF-kappaB binding site.  |  Kim, JY., et al. 2003. Biochem Biophys Res Commun. 310: 904-9. PMID: 14550290
2. Mutations in PLCδ1 associated with hereditary leukonychia display divergent PIP2 hydrolytic function.  |  Nomikos, M., et al. 2016. FEBS J. 283: 4502-4514. PMID: 27783455
3. The phosphoinositide hydrolase phospholipase C delta1 inhibits epithelial-mesenchymal transition and is silenced in colorectal cancer.  |  Xiang, Q., et al. 2019. J Cell Physiol. 234: 13906-13916. PMID: 30618183
4. IQGAP1 activates PLC-δ1 by direct binding and moving along microtubule with DLC-1 to cell surface.  |  Tanaka, M., et al. 2019. FASEB Bioadv. 1: 465-480. PMID: 32123844
5. The Gαh/phospholipase C-δ1 interaction promotes autophagosome degradation by activating the Akt/mTORC1 pathway in metastatic triple-negative breast cancer.  |  Lin, HY., et al. 2020. Aging (Albany NY). 12: 13023-13037. PMID: 32615541
6. Negative self-regulation of transient receptor potential canonical 4 by the specific interaction with phospholipase C-δ1.  |  Ko, J., et al. 2023. Korean J Physiol Pharmacol. 27: 187-196. PMID: 36815258
7. Osmotically evoked PLCδ1-dependent translocation of ΔN-TRPV1 channels in rat supraoptic neurons.  |  Haan, KD., et al. 2023. iScience. 26: 106258. PMID: 36926650
8. A ternary metal binding site in the C2 domain of phosphoinositide-specific phospholipase C-delta1.  |  Essen, LO., et al. 1997. Biochemistry. 36: 2753-62. PMID: 9062102
9. Phosphoinositide-specific phospholipase C delta1 activity toward micellar substrates, inositol 1,2-cyclic phosphate, and other water-soluble substrates: a sequential mechanism and allosteric activation.  |  Wu, Y., et al. 1997. Biochemistry. 36: 11223-33. PMID: 9287165
10. Catalytic domain of phosphoinositide-specific phospholipase C (PLC). Mutational analysis of residues within the active site and hydrophobic ridge of plcdelta1.  |  Ellis, MV., et al. 1998. J Biol Chem. 273: 11650-9. PMID: 9565585

The preferred PLC δ1 antibody is PLC δ1 Antibody (D-7): sc-393464.