PLAP Antibody (8B6) recommended for detection of PLAP of human origin by WB, IP, IF and IHC(P)

PLAP Antibody (8B6): sc-47691

PLAP Antibody (8B6) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-PLAP Antibody (8B6) is a mouse monoclonal IgG2a (kappa light chain) PLAP antibody provided at 200 µg/ml
    • raised against whole cells (Hep-2) expressing PLAP
    • PLAP Antibody (8B6) is recommended for detection of PLAP of human origin by WB, IP, IF and IHC(P)
    • Anti-PLAP Antibody (8B6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLAP (8B6): sc-47691.

SC has two antibodies for human PLAP (G-9 and 8B6). 8B6, is more widely cited, but why is the single band seen by WB not 70kDa (dimer?) while many bands for G-9? Also do you have any blots showing KD with your siRNA to PLAP (sc-38919) for either antibody?

Asked by: SCONE
Thank you for your question. There are three forms of the PLAP protein that can be detected with these antibodies (~67 kD=monomeric,~130 kD=dimeric & ~180 kD=trimeric). We generally see the monomeric or dimeric in our sampels. Since PLAP expression differs in tumor cell lines, what form will be seen varies depending on the samples. Unfortunately we do not have siRNA data for this target with these antibodies, so we are unable to provide that.
Answered by: Tech Service
Date published: 2019-09-26

人胎盘组织裂解液WB的分子量是120KD吗?

Asked by: Hechengjin
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Can PLAP (8B6): sc-47691 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PLAP (8B6): sc-47691 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background.
Date published: 2017-02-03
Rated 5 out of 5 by from Publishable IF data with human placental Publishable IF data with human placental trophoblast cells - SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from WB and IF publishable data with human transfected WB and IF publishable data with human transfected MDCK - SCBT Publication Review
Date published: 2014-03-11
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