Anti-PLAP Antibody (8B6) is a mouse monoclonal IgG2a (kappa light chain) PLAP antibody provided at 200 µg/ml
raised against whole cells (Hep-2) expressing PLAP
PLAP Antibody (8B6) is recommended for detection of PLAP of human origin by WB, IP, IF and IHC(P)
Anti-PLAP Antibody (8B6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLAP (8B6): sc-47691.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PLAP Antibody (8B6) is a high quality monoclonal PLAP antibody (also designated PLAP antibody) suitable for the detection of the PLAP protein of human origin. PLAP Antibody (8B6) is available as both the non-conjugated anti-PLAP antibody form, as well as multiple conjugated forms of anti-PLAP antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SC has two antibodies for human PLAP (G-9 and 8B6). 8B6, is more widely cited, but why is the single band seen by WB not 70kDa (dimer?) while many bands for G-9? Also do you have any blots showing KD with your siRNA to PLAP (sc-38919) for either antibody?
Asked by: SCONE
Thank you for your question. There are three forms of the PLAP protein that can be detected with these antibodies (~67 kD=monomeric,~130 kD=dimeric & ~180 kD=trimeric). We generally see the monomeric or dimeric in our sampels. Since PLAP expression differs in tumor cell lines, what form will be seen varies depending on the samples. Unfortunately we do not have siRNA data for this target with these antibodies, so we are unable to provide that.
Answered by: Tech Service
Date published: 2019-09-26
Asked by: Hechengjin
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21
Can PLAP (8B6): sc-47691 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PLAP (8B6): sc-47691 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 5 out of
Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background.
Date published: 2017-02-03
Rated 5 out of
Publishable IF data with human placentalPublishable IF data with human placental trophoblast cells - SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of
WB and IF publishable data with human transfectedWB and IF publishable data with human transfected MDCK - SCBT Publication Review
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn