PIAS 3 Antibody (C-12): sc-46682

PIAS 3 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects PIAS 3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIAS 3 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIAS 3 plays a crucial role in regulating signal transduction pathways mediated by the IL-6-type family of cytokines, which are essential for gene activation, cell proliferation, and differentiation. Specifically, PIAS 3 functions as a negative regulator of the transcription factor Stat3, which is activated in response to cytokine signaling. By binding to Stat3, PIAS 3 inhibits DNA-binding activity, thereby preventing transcription of target genes crucial for various cellular processes, including inflammation and immune responses. This regulatory mechanism is vital for maintaining cellular homeostasis and preventing excessive activation of Stat3, which can lead to pathological conditions such as cancer and autoimmune diseases. Anti-PIAS 3 antibody (C-12) effectively detects and characterizes PIAS 3, making this reagent invaluable for researchers studying these critical signaling pathways.

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PIAS 3 Antibody (C-12) References:

1. The association and nuclear translocation of the PIAS3-STAT3 complex is ligand and time dependent.  |  Dabir, S., et al. 2009. Mol Cancer Res. 7: 1854-60. PMID: 19903771
2. The enigma of the role of protein inhibitor of activated STAT3 (PIAS3) in the immune response.  |  Yagil, Z., et al. 2010. Trends Immunol. 31: 199-204. PMID: 20181527
3. PIAS3-mediated feedback loops promote chronic colitis-associated malignant transformation.  |  Ma, J., et al. 2018. Theranostics. 8: 3022-3037. PMID: 29896300
4. Post-transcriptional regulation of PIAS3 expression by miR-18a in malignant mesothelioma.  |  He, T., et al. 2018. Mol Oncol. 12: 2124-2135. PMID: 30259640
5. PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma.  |  Bao, W., et al. 2023. Pharmacol Res. 196: 106915. PMID: 37689128
6. PIAS3 acts as a zinc sensor under zinc deficiency and plays an important role in myocardial ischemia/reperfusion injury.  |  Zhao, H., et al. 2024. Free Radic Biol Med. 221: 188-202. PMID: 38750767
7. Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp130-mediated signaling pathway.  |  Akira, S., et al. 1994. Cell. 77: 63-71. PMID: 7512451
8. Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6.  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
9. Cloning and characterization of Gu/RH-II binding protein.  |  Valdez, BC., et al. 1997. Biochem Biophys Res Commun. 234: 335-40. PMID: 9177271
10. Specific inhibition of Stat3 signal transduction by PIAS3.  |  Chung, CD., et al. 1997. Science. 278: 1803-5. PMID: 9388184
11. Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway.  |  Heinrich, PC., et al. 1998. Biochem J. 334 (Pt 2): 297-314. PMID: 9716487
12. Inhibition of Stat1-mediated gene activation by PIAS1.  |  Liu, B., et al. 1998. Proc Natl Acad Sci U S A. 95: 10626-31. PMID: 9724754

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIAS 3 Antibody (C-12) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-PIAS 3 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIAS 3 Antibody (C-12) Specifications

Alternate Product Name: PIAS 3

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 451-619

Epitope Target: PIAS 3

Epitope Species: human

Product Reactivity/Detection: PIAS 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for PIAS 3 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin