PI-9 Antibody (F-12): sc-390561

PI-9 Antibody (F-12) is a mouse monoclonal IgG2a antibody that detects PI-9 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PI-9, also known as proteinase inhibitor-9 or cytoplasmic antiproteinase 3, is a crucial member of the Ovalbumin family of serine proteinase inhibitors (serpins) that plays a significant role in regulating serine proteinase activity across various physiological processes. One of the key functions of PI-9 is inhibiting granzyme B, a serine protease essential for inducing apoptosis in target cells during immune responses. By preventing granzyme B-mediated apoptosis, PI-9 serves as a protective mechanism for cytotoxic lymphocytes, endothelial cells, and epithelial cells, particularly in inflammation and tumorigenesis. The presence of PI-9 in cell nuclei highlights PI-9′s importance in modulating apoptotic pathways, as granzyme B can translocate to the nucleus and promote DNA degradation. Furthermore, PI-9 is rapidly induced by estrogen and upregulated in response to inflammatory stimuli, which enhances PI-9′s protective role against apoptosis during immune responses. The expression of PI-9 in various human and murine tumors underscores PI-9′s potential significance in cancer biology, making anti-PI-9 antibody (F-12) a valuable tool for researchers investigating the intricate balance between apoptosis and cell survival in both normal and pathological conditions.

The preferred PI-9 antibody is PI-9 Antibody (F-6): sc-390501.