PGC-1 beta Antibody (E-9) recommended for detection of PGC-1β of mouse and rat origin by WB, IP, IF and ELISA

PGC-1β Antibody (E-9): sc-373771

PGC-1β Antibody (E-9) is rated 4.9 out of 5 by 12.
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Datasheets

    • Anti-PGC-1β Antibody (E-9) is a mouse monoclonal IgG2a (kappa light chain) PGC-1β antibody provided at 200 µg/ml
    • raised against amino acids 452-593 mapping within an internal region of PGC-1β of mouse origin
    • Anti-PGC-1 beta Antibody (E-9) is recommended for detection of PGC-1β of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-PGC-1 beta Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-373771 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PGC-1β (E-9): sc-373771.
Submit a review for this product and receive 15 CruzCredits

Hello, I'm interested in your PGC-1beta antibody (E9);sc-373771. Can I have the 10ug free sample?

Asked by: Tomoki
Thank you for the question. Please contact us by email to customercare @ scbt.com and provide your full shipping address and we will be happy to send you a PGC-1β (E-9): sc-373771 Sample size.
Answered by: Technical Support Europe
Date published: 2017-12-29

I am using human epithelial cells. But i m not able to get a proper band with this antibody. Can you suggest a possible protocol for the same for a human cell line. Kindly help me out with this issue?

Asked by: Aloysius
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. Alternatively, you can contact our local distributor in India, https://www.scbt.com/scbt/resources/distributors. Here is a link to the recommended protocol for this antibody. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Tech Service
Date published: 2017-02-17

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-19
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Rated 5 out of 5 by from very good for western It detects a strong western band for mouse protein from tissue extract at 1:1000 dilution, 3% BSA, PBST.
Date published: 2017-11-03
Rated 5 out of 5 by from Great antibody for WB I test this antibody with Liver cells and the results are very nice, strong, clear signal; 1:500
Date published: 2017-10-29
Rated 5 out of 5 by from Good features I bought this a week ago, I did Western Blot(dilution 1:100), but one band is unclear
Date published: 2017-07-18
Rated 5 out of 5 by from Works well in cultured myotube Produced great WB data in C2C12 myotube, no non-specific bands.
Date published: 2017-05-11
Rated 4 out of 5 by from Detects the protein well in mouse muscle WB, 5% milk in TBST. 1:1000. Though there are some non-specific bands, the target protein is easily identifiable. Better results were obtained from cultured myotubes.
Date published: 2017-05-09
Rated 5 out of 5 by from Great product Very nice western blot data; strong, clear signal; also positive IHC staining
Date published: 2017-02-03
Rated 5 out of 5 by from Good WB data with Mouse liver I bought this antibody and used it 1:1000 for Western Blotting,I got the wonderful band
Date published: 2017-01-13
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of PGC-1β expression in KNRK whole cell lysate.
Date published: 2017-01-12
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