PGAM1/4 Antibody (D-5): sc-365677

PGAM1/4 Antibody (D-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects PGAM1/4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PGAM1/4 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PGAM1/4 protein plays a crucial role in cellular metabolism, specifically in the interconversion of 3-phosphoglycerate and 2-phosphoglycerate, which are key intermediates in glycolysis and gluconeogenesis. This enzymatic activity is vital for maintaining energy homeostasis and facilitating efficient glucose use, particularly in tissues with high metabolic demands such as heart and skeletal muscle. The PGAM family consists of several isozymes, including PGAM1 and PGAM2, which can form homodimers or heterodimers, influencing functional properties and tissue distribution. Notably, PGAM4, previously thought to be a pseudogene, has been shown to encode a functional protein, highlighting the evolutionary significance of this protein family. Anti-PGAM1/4 antibody (D-5) effectively detects these proteins across different species, making PGAM1/4 (D-5) monoclonal antibody an invaluable tool for researchers studying metabolic pathways and their implications in health and disease.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PGAM1/4 Antibody (D-5) References:

1. Mouse phosphoglycerate mutase M and B isozymes: cDNA cloning, enzyme activity assay and mapping.  |  Zhang, J., et al. 2001. Gene. 264: 273-9. PMID: 11250083
2. Evolution of the phosphoglycerate mutase processed gene in human and chimpanzee revealing the origin of a new primate gene.  |  Betrán, E., et al. 2002. Mol Biol Evol. 19: 654-63. PMID: 11961099
3. A p21-activated kinase-controlled metabolic switch up-regulates phagocyte NADPH oxidase.  |  Shalom-Barak, T. and Knaus, UG. 2002. J Biol Chem. 277: 40659-65. PMID: 12189148
4. Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis.  |  Saavedra, E., et al. 2005. FEBS J. 272: 1767-83. PMID: 15794763
5. Characterization of the first described mutation of human red blood cell phosphoglycerate mutase.  |  de Atauri, P., et al. 2005. Biochim Biophys Acta. 1740: 403-10. PMID: 15949708
6. Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling.  |  Evans, MJ., et al. 2005. Nat Biotechnol. 23: 1303-7. PMID: 16200062
7. Proteomic analysis of secreted proteins of non-small cell lung cancer.  |  Huang, LJ., et al. 2006. Ai Zheng. 25: 1361-7. PMID: 17094902
8. Mechanistic and structural requirements for active site labeling of phosphoglycerate mutase by spiroepoxides.  |  Evans, MJ., et al. 2007. Mol Biosyst. 3: 495-506. PMID: 17579775
9. Screening cellular proteins binding to the core region of hepatitis C virus RNA genome with digoxin-labeled nucleic acids.  |  Su, HX., et al. 2007. Intervirology. 50: 303-9. PMID: 17622790
10. Targeting PGAM1 in cancer: An emerging therapeutic opportunity.  |  Yang, GJ., et al. 2022. Eur J Med Chem. 244: 114798. PMID: 36215859
11. Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1.  |  Luo, JQ., et al. 2023. Cell Death Dis. 14: 502. PMID: 37542027
12. PGAM1 Inhibition Promotes HCC Ferroptosis and Synergizes with Anti-PD-1 Immunotherapy.  |  Zheng, Y., et al. 2023. Adv Sci (Weinh). 10: e2301928. PMID: 37705495

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PGAM1/4 Antibody (D-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-PGAM1/4 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PGAM1/4 Antibody (D-5) Specifications

Alternate Product Name: PGAM1/4

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 116-147

Epitope Target: PGAM1

Epitope Species: human

Product Reactivity/Detection: PGAM1 of mouse, rat and human origin, and PGAM4 of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for PGAM1/4 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PGAM1 antibody is PGAM1 Antibody (6): sc-130334.