PFK-2/PFKFB2 Antibody (G-7): sc-515528

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP, and the most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

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PFK-2/PFKFB2 Antibody (G-7) References:

1. Molecular cloning of a cDNA encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from liver of Sparus aurata: nutritional regulation of enzyme expression.  |  Metón, I., et al. 1999. Biochim Biophys Acta. 1444: 153-65. PMID: 10023046
2. Improved metabolic stimulus for glucose-induced insulin secretion through GK and PFK-2/FBPase-2 coexpression in insulin-producing RINm5F cells.  |  Baltrusch, S., et al. 2006. Endocrinology. 147: 5768-76. PMID: 16980436
3. A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells.  |  Arden, C., et al. 2008. Biochem J. 411: 41-51. PMID: 18039179
4. Androgen stimulates glycolysis for de novo lipid synthesis by increasing the activities of hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 in prostate cancer cells.  |  Moon, JS., et al. 2011. Biochem J. 433: 225-33. PMID: 20958264
5. Fructose-2,6-bisphosphate in control of hepatic gluconeogenesis. From metabolites to molecular genetics.  |  Pilkis, SJ., et al. 1990. Diabetes Care. 13: 582-99. PMID: 2162755
6. Upregulation of heart PFK-2/FBPase-2 isozyme in skeletal muscle after persistent contraction.  |  Rovira, J., et al. 2012. Pflugers Arch. 463: 603-13. PMID: 22241065
7. Glucagon induces translocation of glucokinase from the cytoplasm to the nucleus of hepatocytes by transfer between 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2 and the glucokinase regulatory protein.  |  Cullen, KS., et al. 2014. Biochim Biophys Acta. 1843: 1123-34. PMID: 24566088
8. The role of 6-phosphofructo-2-kinase (PFK-2)/fructose 2,6-bisphosphatase (FBPase-2) in metabolic reprogramming of cancer cells.  |  Kocemba, KA., et al. 2016. Postepy Hig Med Dosw (Online). 70: 938-950. PMID: 27668646
9. Tolbutamide inhibits glucagon-induced phosphorylation of 6PF-2-K/Fru-2,6-P2ase in rat hepatocytes.  |  Ayame, H., et al. 1995. Am J Physiol. 268: E391-6. PMID: 7900785
10. Preparation of monoclonal antibodies against chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and their effects on enzyme activities.  |  Li, T., et al. 1996. Sci China C Life Sci. 39: 342-9. PMID: 9388835

The preferred PFK-2/PFKFB2 antibody is PFK-2/PFKFB2 Antibody (D-1): sc-377416.