Anti-PEPT1 Antibody (E-3) is a mouse monoclonal IgG1 (kappa light chain) PEPT1 antibody provided at 200 µg/ml
raised against amino acids 366-600 of PEPT1 of human origin
Anti-PEPT1 Antibody (E-3) is recommended for detection of PEPT1 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-PEPT1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PEPT1 (E-3): sc-373742.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PEPT1 Antibody (E-3) is a high quality monoclonal PEPT1 antibody (also designated PEPT1 antibody) suitable for the detection of the PEPT1 protein of mouse, rat and human origin. PEPT1 Antibody (E-3) is available as both the non-conjugated anti-PEPT1 antibody form, as well as multiple conjugated forms of anti-PEPT1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In mammalian small intestine, the proton-coupled peptide transporter (PEPT) is responsible for the absorption of small peptides arising from digestion of dietary proteins. PEPT1, a hydrogen ion/peptide cotransporter, transports dipeptides and tripeptides, but not free amino acids or peptides with more than three amino acid residues. Its driving force for uphill transport requires proton binding and the presence of an inside-negative membrane potential. PEPT1 is 708 amino acid protein that contains 12 putative membrane-spanning domains. PEPT1 is expressed in Caco-2 cells. PEPT1 seems to play important roles in nutritional and pharmacological therapies. The mammalian kidney expresses a proton-coupled peptide transporter, PEPT2, that is responsible for the absorption of small peptides, as well as beta-lactam antibiotics and other peptide-like drugs, from the tubular filtrate. The gene which encodes PEPT1 maps to human chromosome 13q33-q34.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. This antibody is provided at 200 µg in 1 ml.
Answered by: Technical Support
Date published: 2018-12-19
How much sample do you recommend loading on the gel for Western blot detection using PEPT1 (E-3): sc-373742?
Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
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