PEPT1 Antibody (E-3) recommended for detection of PEPT1 of mouse, rat and human origin by WB, IP, IF and ELISA

PEPT1 Antibody (E-3): sc-373742

PEPT1 Antibody (E-3) is rated 4.8 out of 5 by 5.
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Datasheets

    • Anti-PEPT1 Antibody (E-3) is a mouse monoclonal IgG1 (kappa light chain) PEPT1 antibody provided at 200 µg/ml
    • raised against amino acids 366-600 of PEPT1 of human origin
    • Anti-PEPT1 Antibody (E-3) is recommended for detection of PEPT1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-PEPT1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PEPT1 (E-3): sc-373742.
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What volume is supplied with sc-373742?

Asked by: awmoran
Thank you for your question. This antibody is provided at 200 µg in 1 ml.
Answered by: Technical Support
Date published: 2018-12-19

How much sample do you recommend loading on the gel for Western blot detection using PEPT1 (E-3): sc-373742?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for IF (P) It worked for immunofluorescence staining in paraffin sections of human and murine intestine.
Date published: 2018-03-28
Rated 5 out of 5 by from 质量很好很稳定 Western blot检测大鼠小肠PEPT1的蛋白条带,下面出现了2条杂带,上面一条目的蛋白分子条带也出来了
Date published: 2017-10-16
Rated 5 out of 5 by from Excellent This antibody is great in western blot analysis of PEPT1 expression in CCRF-CEM whole cell lysate.
Date published: 2017-01-24
Rated 4 out of 5 by from Worked well with porcine tissues Worked with protein extracted from porcine intestinal mucosa. Way less messy than polyclonal
Date published: 2016-12-06
Rated 5 out of 5 by from Produced nice Western blot data of PEPT1 Produced nice Western blot data of PEPT1 expression in KNRK whole cell lysate. -SCBT QC
Date published: 2013-06-17
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