PEPCK Antibody (E-1)recommended for detection of PEPCK-M and PEPCK-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PEPCK Antibody (E-1): sc-271204

PEPCK Antibody (E-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • PEPCK Antibody (E-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 341-640 mapping at the C-terminus of PEPCK of human origin
    • recommended for detection of PEPCK-M and PEPCK-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See PEPCK (F-3): sc-271029 for PEPCK antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using PEPCK (E-1): sc-271204 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good specificity in WB in human liver tissue Good specificity in WB in human liver tissue extract. -SCBT QC
Date published: 2015-05-31
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmic, nuclear and nucleolar staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Robust signal in WB in A Robust signal in WB in A-431 whole cell lysate. -SCBT QC
Date published: 2015-02-18
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