PEPCK/PCK1/PCK2 Antibody (F-3): sc-271029

PEPCK Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody designed to target Phosphoenolpyruvate carboxykinase (PEPCK), also known as PCK1 and PCK2. PEPCK monoclonal antibody (F-3) is raised against amino acids 341-640 at the C-terminus of the mitochondrial form of human PEPCK (PEPCK-M), ensuring precise recognition of both PEPCK-M and the cytosolic form (PEPCK-C) across mouse, rat, and human species. PEPCK is a crucial enzyme located in both the cytosol and mitochondria of liver cells, where PEPCK plays a vital role in gluconeogenesis, the metabolic pathway responsible for producing glucose from non-carbohydrate sources. The dual localization of PEPCK-C and PEPCK-M allows for efficient regulation of glucose synthesis in response to hormonal signals such as glucagon, glucocorticoids, and insulin, which modulate expression levels. Dysregulation of PEPCK activity is closely associated with metabolic disorders, including type 2 diabetes and obesity, making PEPCK a significant target for therapeutic research. Anti-PEPCK antibody (F-3) is versatile in applications, suitable for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Additionally, PEPCK (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing flexibility for diverse experimental needs. By enabling precise detection and study of PEPCK in different cellular compartments, anti-PEPCK antibody (F-3) supports advanced research into metabolic regulation and the development of potential treatments for insulin resistance and related conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PEPCK/PCK1/PCK2 Antibody (F-3) References:

1. [Novel aspects in the mechanisms of steroid diabetes and the regulation of hepatic glucose production by insulin and steroids].  |  Barthel, A., et al. 2003. Med Klin (Munich). 98: 283-6. PMID: 12721674
2. Identification of a novel variant in the phosphoenolpyruvate carboxykinase gene promoter in Japanese patients with type 2 diabetes.  |  Horikawa, Y., et al. 2003. Horm Metab Res. 35: 308-12. PMID: 12916001
3. Novel concepts in insulin regulation of hepatic gluconeogenesis.  |  Barthel, A. and Schmoll, D. 2003. Am J Physiol Endocrinol Metab. 285: E685-92. PMID: 12959935
4. Sustained peripheral expression of transgene adiponectin offsets the development of diet-induced obesity in rats.  |  Shklyaev, S., et al. 2003. Proc Natl Acad Sci U S A. 100: 14217-22. PMID: 14617771
5. AMP-activated protein kinase regulates PEPCK gene expression by direct phosphorylation of a novel zinc finger transcription factor.  |  Inoue, E. and Yamauchi, J. 2006. Biochem Biophys Res Commun. 351: 793-9. PMID: 17097062
6. Insulin and other regulatory factors modulate the growth and the phosphoenolpyruvate carboxykinase (PEPCK) activity of primary rabbit kidney proximal tubule cells in serum free medium.  |  Wang, Y. and Taub, M. 1991. J Cell Physiol. 147: 374-82. PMID: 1710231
7. Structures of rat cytosolic PEPCK: insight into the mechanism of phosphorylation and decarboxylation of oxaloacetic acid.  |  Sullivan, SM. and Holyoak, T. 2007. Biochemistry. 46: 10078-88. PMID: 17685635
8. Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription.  |  O'Brien, RM., et al. 1990. Science. 249: 533-7. PMID: 2166335
9. Insulin decreases H4IIE cell PEPCK mRNA by a mechanism that does not involve cAMP.  |  Beale, EG., et al. 1986. Diabetes. 35: 546-9. PMID: 3007246

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PEPCK/PCK1/PCK2 Antibody (F-3) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-PEPCK/PCK1/PCK2 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PEPCK/PCK1/PCK2 Antibody (F-3) Specifications

Alternate Product Name: PEPCK

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 341-640

Epitope Target: PEPCK-M

Epitope Species: human

Product Reactivity/Detection: PEPCK-M and PEPCK-C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for PEPCK/PCK1/PCK2 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin