PDX-1 Antibody (E-5): sc-390808

PDX-1 Antibody (E-5) is a mouse monoclonal IgG2b antibody that detects pancreatic duodenal homeobox-1 (PDX-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDX-1 is a crucial transcription factor composed of 282 amino acids, primarily found in pancreatic beta-cells, where PDX-1 plays a vital role in regulating pancreatic islet development and insulin gene transcription. PDX-1 (E-5) antibody′s target can bind to specific DNA sequences and form heterodimers with other proteins, such as PBX, underscoring PDX-1′s importance in transcriptional regulation of insulin, which is essential for glucose homeostasis. Additionally, PDX-1 undergoes post-translational modifications, including phosphorylation by the SAPK2 pathway in response to high glucose levels, which enhances PDX-1′s activity and promotes nuclear translocation in beta-cells. This regulatory mechanism is critical, as mutations in PDX-1 can lead to severe metabolic disorders, including maturity-onset diabetes of the young and pancreatic agenesis. The gene encoding PDX-1 is located on human chromosome 13q12.1, and PDX-1 expression is tightly controlled by various factors, including HNF-3β, HNF-1α, and SP1, as well as glucagon-like peptide, which modulates intracellular cAMP levels and subsequently influences PDX-1 transcription. With PDX-1′s significant role in insulin regulation and potential implications in diabetes, anti-PDX-1 antibody (E-5) serves as an invaluable tool for researchers studying pancreatic function and related metabolic diseases.

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PDX-1 Antibody (E-5) References:

1. Regulation of pdx-1 gene expression.  |  Melloul, D., et al. 2002. Diabetes. 51 Suppl 3: S320-5. PMID: 12475770
2. PDX-1 and the pancreas.  |  Ashizawa, S., et al. 2004. Pancreas. 28: 109-20. PMID: 15028942
3. Pdx-1 is not sufficient for repression of proglucagon gene transcription in islet or enteroendocrine cells.  |  Flock, G., et al. 2005. Endocrinology. 146: 441-9. PMID: 15471960
4. Role of PDX-1 and MafA as a potential therapeutic target for diabetes.  |  Kaneto, H., et al. 2007. Diabetes Res Clin Pract. 77 Suppl 1: S127-37. PMID: 17449132
5. Pdx-1 or Pdx-1-VP16 protein transduction induces beta-cell gene expression in liver-stem WB cells.  |  Delisle, JC., et al. 2009. BMC Res Notes. 2: 3. PMID: 19134185
6. ATF3 inhibits PDX-1-stimulated transactivation.  |  Kim, WH., et al. 2011. Biochem Biophys Res Commun. 414: 681-7. PMID: 21986529
7. The Phosphorylation of PDX-1 by Protein Kinase CK2 Is Crucial for Its Stability.  |  Klein, S., et al. 2016. Pharmaceuticals (Basel). 10: PMID: 28036027
8. Epigenetic Regulation of PDX-1 in Type 2 Diabetes Mellitus.  |  Liu, J., et al. 2021. Diabetes Metab Syndr Obes. 14: 431-442. PMID: 33564250
9. TAZ promotes PDX1-mediated insulinogenesis.  |  Jeong, MG., et al. 2022. Cell Mol Life Sci. 79: 186. PMID: 35279781
10. Regulation of Pdx1 by oxidative stress and Nrf2 in pancreatic beta-cells.  |  Baumel-Alterzon, S. and Scott, DK. 2022. Front Endocrinol (Lausanne). 13: 1011187. PMID: 36187092

The preferred PDX-1 antibody is PDX-1 Antibody (B-11): sc-390792.