PDX-1 Antibody (B-11): sc-390792

PDX-1 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDX-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDX-1 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The pancreatic duodenal homeobox-1 protein (PDX-1), also known as insulin promoter factor 1, is a crucial 282 amino acid transcription factor that plays a vital role in the development of pancreatic islets and the regulation of insulin gene transcription in beta-cells. PDX-1 is predominantly expressed in the pancreas and duodenum during adulthood, although PDX-1 is present in all cells during early development. PDX-1 function is essential for maintaining glucose homeostasis, as PDX-1 regulates the expression of genes involved in insulin production. PDX-1 interacts with several key proteins, including HNF-3β, HNF-1α, and SP1, which enhance transcriptional activity. Additionally, PDX-1 is modulated by glucagon-like peptide, leading to increased intracellular cAMP levels that activate protein kinase A, further promoting PDX-1 transcription and translocation to the nuclei of beta-cells. Notably, mutations in PDX-1 can result in serious conditions such as maturity-onset diabetes of the young and pancreatic agenesis, highlighting PDX-1′s critical role in metabolic health. PDX-1 is located on human chromosome 13q12.1, underscoring PDX-1′s importance in both developmental biology and disease.

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PDX-1 Antibody (B-11) References:

1. Missense mutations in the insulin promoter factor-1 gene predispose to type 2 diabetes.  |  Macfarlane, WM., et al. 1999. J Clin Invest. 104: R33-9. PMID: 10545530
2. Defective mutations in the insulin promoter factor-1 (IPF-1) gene in late-onset type 2 diabetes mellitus.  |  Hani, EH., et al. 1999. J Clin Invest. 104: R41-8. PMID: 10545531
3. The homeodomain of PDX-1 mediates multiple protein-protein interactions in the formation of a transcriptional activation complex on the insulin promoter.  |  Ohneda, K., et al. 2000. Mol Cell Biol. 20: 900-11. PMID: 10629047
4. Glucose modulation of insulin mRNA levels is dependent on transcription factor PDX-1 and occurs independently of changes in intracellular Ca2+.  |  Macfarlane, WM., et al. 2000. Diabetes. 49: 418-23. PMID: 10868963
5. A pancreatic beta -cell-specific enhancer in the human PDX-1 gene is regulated by hepatocyte nuclear factor 3beta (HNF-3beta), HNF-1alpha, and SPs transcription factors.  |  Ben-Shushan, E., et al. 2001. J Biol Chem. 276: 17533-40. PMID: 11278466
6. Glucagon-like peptide-1 causes pancreatic duodenal homeobox-1 protein translocation from the cytoplasm to the nucleus of pancreatic beta-cells by a cyclic adenosine monophosphate/protein kinase A-dependent mechanism.  |  Wang, X., et al. 2001. Endocrinology. 142: 1820-7. PMID: 11316746
7. PDX-1: A Promising Therapeutic Target to Reverse Diabetes.  |  Zhang, Y., et al. 2022. Biomolecules. 12: PMID: 36551213
8. Comprehensive Search for GPCR Compounds which Can Enhance MafA and/or PDX-1 Expression Levels Using a Small Molecule Compound Library.  |  Kaneto, H., et al. 2023. J Diabetes Res. 2023: 8803172. PMID: 37720599
9. Repression of latent NF-κB enhancers by PDX1 regulates β cell functional heterogeneity.  |  Weidemann, BJ., et al. 2024. Cell Metab. 36: 90-102.e7. PMID: 38171340
10. Localization of human homeodomain transcription factor insulin promoter factor 1 (IPF1) to chromosome band 13q12.1.  |  Stoffel, M., et al. 1995. Genomics. 28: 125-6. PMID: 7590740
11. Isolation, characterization, and chromosomal mapping of the human insulin promoter factor 1 (IPF-1) gene.  |  Inoue, H., et al. 1996. Diabetes. 45: 789-94. PMID: 8635654
12. The p38/reactivating kinase mitogen-activated protein kinase cascade mediates the activation of the transcription factor insulin upstream factor 1 and insulin gene transcription by high glucose in pancreatic beta-cells.  |  Macfarlane, WM., et al. 1997. J Biol Chem. 272: 20936-44. PMID: 9252422

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDX-1 Antibody (B-11) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-PDX-1 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDX-1 Antibody (B-11) Specifications

Alternate Product Name: Pancreatic and Duodenal Homeobox 1

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-140 deletion 20-56

Epitope Target: PDX-1

Epitope Species: human

Product Reactivity/Detection: PDX-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for PDX-1 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent