PDE1C Antibody (G-7): sc-376474

PDE1C Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDE1C protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PDE1C antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE1C protein, also known as phosphodiesterase 1C or HCAM3, plays a crucial role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are vital second messengers involved in numerous signaling pathways. PDE1C protein is particularly important due to calmodulin-dependent activity, which is stimulated by a calcium-calmodulin complex, allowing hydrolysis of cAMP to 5′AMP and cGMP to 5′GMP. PDE1C is widely expressed, with two isoforms—PDE1C1 and PDE1C2—resulting from alternative splicing at the C-terminus. While both isoforms are present at low levels throughout the body, PDE1C2 is predominantly found in the brain and heart, whereas PDE1C1 is expressed mainly in the brain, heart, and lung. Understanding PDE1C distribution and function is essential for elucidating roles in various physiological processes and potential implications in diseases related to dysregulated cyclic nucleotide signaling.

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PDE1C Antibody (G-7) References:

1. Characterization of cAMP degradation by phosphodiesterases in the accessory olfactory system.  |  Cherry, JA. and Pho, V. 2002. Chem Senses. 27: 643-52. PMID: 12200345
2. Cyclic GMP phosphodiesterases and regulation of smooth muscle function.  |  Rybalkin, SD., et al. 2003. Circ Res. 93: 280-91. PMID: 12933699
3. Cyclic nucleotide phosphodiesterases (PDEs) in human osteoblastic cells; the effect of PDE inhibition on cAMP accumulation.  |  Ahlström, M., et al. 2005. Cell Mol Biol Lett. 10: 305-19. PMID: 16010295
4. Inhibition of phosphodiesterase 1 augments the pulmonary vasodilator response to inhaled nitric oxide in awake lambs with acute pulmonary hypertension.  |  Evgenov, OV., et al. 2006. Am J Physiol Lung Cell Mol Physiol. 290: L723-L729. PMID: 16284211
5. Subcellular localization and regulation of type-1C and type-5 phosphodiesterases.  |  Dolci, S., et al. 2006. Biochem Biophys Res Commun. 341: 837-46. PMID: 16455054
6. Characterization of sensory neuron subpopulations selectively expressing green fluorescent protein in phosphodiesterase 1C BAC transgenic mice.  |  Torsney, C., et al. 2006. Mol Pain. 2: 17. PMID: 16681857
7. Expression and activity of cAMP phosphodiesterase isoforms in pulmonary artery smooth muscle cells from patients with pulmonary hypertension: role for PDE1.  |  Murray, F., et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292: L294-303. PMID: 16980375
8. Cyclic nucleotide phosphodiesterase PDE1C1 in human cardiac myocytes.  |  Vandeput, F., et al. 2007. J Biol Chem. 282: 32749-57. PMID: 17726023
9. A dominant variant in the PDE1C gene is associated with nonsyndromic hearing loss.  |  Wang, L., et al. 2018. Hum Genet. 137: 437-446. PMID: 29860631
10. Multiprotein Complex With TRPC (Transient Receptor Potential-Canonical) Channel, PDE1C (Phosphodiesterase 1C), and A2R (Adenosine A2 Receptor) Plays a Critical Role in Regulating Cardiomyocyte cAMP and Survival.  |  Zhang, Y., et al. 2018. Circulation. 138: 1988-2002. PMID: 29871977
11. The calmodulin-dependent phosphodiesterase gene PDE1C encodes several functionally different splice variants in a tissue-specific manner.  |  Yan, C., et al. 1996. J Biol Chem. 271: 25699-706. PMID: 8810348
12. Calmodulin-stimulated cyclic nucleotide phosphodiesterase (PDE1C) is induced in human arterial smooth muscle cells of the synthetic, proliferative phenotype.  |  Rybalkin, SD., et al. 1997. J Clin Invest. 100: 2611-21. PMID: 9366577

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDE1C Antibody (G-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PDE1C Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDE1C Antibody (G-7) Specifications

Alternate Product Name: PDE1C

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 9-35

Epitope Target: PDE1C

Epitope Species: human

Product Reactivity/Detection: PDE1C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PDE1C Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin