PDE10A Antibody (G-7): sc-515023
- PDE10A Antibody (G-7) is a mouse monoclonal IgG2a κ PDE10A antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 82-300 mapping near the N-terminus of PDE10A of human origin
- PDE10A Antibody (G-7) is recommended for detection of PDE10A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PDE10A Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PDE10A Antibody (G-7) for WB applications. These reagents are now offered in bundles with PDE10A Antibody (G-7) (see ordering information below).
PDE10A Antibody (G-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDE10A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDE10A (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE10A, or phosphodiesterase 10A, is a striatum-enriched dual-substrate phosphodiesterase that plays a crucial role in signal transduction by modulating cyclic nucleotides levels, specifically cGMP and cAMP. This regulation maintains medium spiny neurons′ excitability in the striatum, essential for motor control and cognitive functions. PDE10A protein is predominantly localized in the cytoplasm, where PDE10A hydrolyzes cyclic nucleotides to their corresponding nucleoside 5-prime monophosphates, effectively terminating cGMP- and cAMP-mediated signaling pathways. PDE10A protein is highly expressed in the putamen and caudate nuclei of the brain and testis, with moderate levels found in the pituitary gland, thalamus, and cerebellum. PDE10A structure includes an N-terminal regulatory domain and a C-terminal catalytic domain, which features two putative divalent metal binding sites, highlighting its complex functionality. Alternative splicing results in two isoforms of PDE10A, further diversifying its biological roles.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDE10A Antibody (G-7) References:
- Mutant huntingtin affects the rate of transcription of striatum-specific isoforms of phosphodiesterase 10A. | Hu, H., et al. 2004. Eur J Neurosci. 20: 3351-63. PMID: 15610167
- PDE10A inhibition reverses subchronic PCP-induced deficits in attentional set-shifting in rats. | Rodefer, JS., et al. 2005. Eur J Neurosci. 21: 1070-6. PMID: 15787711
- Cellular and subcellular localization of PDE10A, a striatum-enriched phosphodiesterase. | Xie, Z., et al. 2006. Neuroscience. 139: 597-607. PMID: 16483723
- Genetic deletion of the striatum-enriched phosphodiesterase PDE10A: evidence for altered striatal function. | Siuciak, JA., et al. 2006. Neuropharmacology. 51: 374-85. PMID: 16769090
- Inhibition of the striatum-enriched phosphodiesterase PDE10A: a novel approach to the treatment of psychosis. | Siuciak, JA., et al. 2006. Neuropharmacology. 51: 386-96. PMID: 16780899
- Immunohistochemical localization of phosphodiesterase 10A in multiple mammalian species. | Coskran, TM., et al. 2006. J Histochem Cytochem. 54: 1205-13. PMID: 16864896
- Development of a cell-based assay for screening of phosphodiesterase 10A (PDE10A) inhibitors using a stable recombinant HEK-293 cell line expressing high levels of PDE10A. | Bora, RS., et al. 2008. Biotechnol Appl Biochem. 49: 129-34. PMID: 17640173
- Behavioral characterization of mice deficient in the phosphodiesterase-10A (PDE10A) enzyme on a C57/Bl6N congenic background. | Siuciak, JA., et al. 2008. Neuropharmacology. 54: 417-27. PMID: 18061215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDE10A Antibody (G-7) | sc-515023 | 200 µg/ml | $316.00 | |||
PDE10A Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-531269 | 200 µg Ab; 10 µg BP | $354.00 | |||
PDE10A Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-524908 | 200 µg Ab, 40 µg BP | $354.00 | |||
PDE10A Antibody (G-7): m-IgG2a BP-HRP Bundle | sc-547746 | 200 µg Ab; 10 µg BP | $354.00 | |||
PDE10A Antibody (G-7) AC | sc-515023 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PDE10A Antibody (G-7) HRP | sc-515023 HRP | 200 µg/ml | $316.00 | |||
PDE10A Antibody (G-7) FITC | sc-515023 FITC | 200 µg/ml | $330.00 | |||
PDE10A Antibody (G-7) PE | sc-515023 PE | 200 µg/ml | $343.00 | |||
PDE10A Antibody (G-7) Alexa Fluor® 488 | sc-515023 AF488 | 200 µg/ml | $357.00 | |||
PDE10A Antibody (G-7) Alexa Fluor® 546 | sc-515023 AF546 | 200 µg/ml | $357.00 | |||
PDE10A Antibody (G-7) Alexa Fluor® 594 | sc-515023 AF594 | 200 µg/ml | $357.00 | |||
PDE10A Antibody (G-7) Alexa Fluor® 647 | sc-515023 AF647 | 200 µg/ml | $357.00 | |||
PDE10A Antibody (G-7) Alexa Fluor® 680 | sc-515023 AF680 | 200 µg/ml | $357.00 | |||
PDE10A Antibody (G-7) Alexa Fluor® 790 | sc-515023 AF790 | 200 µg/ml | $357.00 |