PC-PLD2 Antibody (B-3): sc-515744

PC-PLD2 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PC-PLD2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PC-PLD2 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PC-PLD2 plays a crucial role in cellular signaling and membrane dynamics, primarily localized to the plasma membrane, where PC-PLD2 is essential for the formation of endocytotic vesicles. This localization is significant as PC-PLD2 participates in the regulation of macrophage phagocytosis and the stimulation of phosphatidic acid production, which is vital for various cellular processes, including cell growth and migration. Additionally, PC-PLD2 is activated by G protein-coupled receptors and receptor tyrosine kinases, linking PC-PLD2 to important signaling pathways such as the stimulation of extracellular signal-regulated kinase phosphorylation and interleukin-8 secretion in bronchial epithelial cells. The interaction of PC-PLD2 with other proteins, such as tubulin, can modulate PC-PLD2 activity, highlighting PC-PLD2′s role in complex cellular mechanisms. Furthermore, PC-PLD2′s ability to enhance protein kinase C zeta activity through direct interaction, independent of lipase activity, underscores PC-PLD2′s multifaceted role in cellular regulation. Understanding PC-PLD2 function and dynamics is essential for elucidating PC-PLD2′s contributions to cellular physiology and potential implications in disease processes, including cancer.

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PC-PLD2 Antibody (B-3) References:

1. Regulation of phospholipase D by phosphorylation-dependent mechanisms.  |  Houle, MG. and Bourgoin, S. 1999. Biochim Biophys Acta. 1439: 135-49. PMID: 10425391
2. Signalling roles of mammalian phospholipase D1 and D2.  |  Cockcroft, S. 2001. Cell Mol Life Sci. 58: 1674-87. PMID: 11706993
3. Generation of choline for acetylcholine synthesis by phospholipase D isoforms.  |  Zhao, D., et al. 2001. BMC Neurosci. 2: 16. PMID: 11734063
4. Involvement of phospholipases D1 and D2 in sphingosine 1-phosphate-induced ERK (extracellular-signal-regulated kinase) activation and interleukin-8 secretion in human bronchial epithelial cells.  |  Wang, L., et al. 2002. Biochem J. 367: 751-60. PMID: 12149127
5. Serine/threonine protein kinases synergistically regulate phospholipase D1 and 2 and secretion in RBL-2H3 mast cells.  |  Chahdi, A., et al. 2002. Mol Immunol. 38: 1269-76. PMID: 12217394
6. Transmodulation between phospholipase D and c-Src enhances cell proliferation.  |  Ahn, BH., et al. 2003. Mol Cell Biol. 23: 3103-15. PMID: 12697812
7. Transcriptional repression of cyclin-dependent kinase inhibitor p21 gene by phospholipase D1 and D2.  |  Kwun, HJ., et al. 2003. FEBS Lett. 544: 38-44. PMID: 12782287
8. Phospholipases D1 and D2 coordinately regulate macrophage phagocytosis.  |  Iyer, SS., et al. 2004. J Immunol. 173: 2615-23. PMID: 15294978
9. Novel functions of the phospholipase D2-Phox homology domain in protein kinase Czeta activation.  |  Kim, JH., et al. 2005. Mol Cell Biol. 25: 3194-208. PMID: 15798205
10. Loss of phospholipase D2 impairs VEGF-induced angiogenesis.  |  Lee, CS., et al. 2016. BMB Rep. 49: 191-6. PMID: 26818087
11. Brain ischemia decreases phosphatidylcholine-phospholipase D but not phosphatidylinositol-phospholipase C in rats.  |  Nishida, A., et al. 1994. Stroke. 25: 1247-51. PMID: 8202988
12. Activation of phospholipase D by ras proteins is independent of protein kinase C.  |  del Peso, L., et al. 1996. J Cell Biochem. 61: 599-608. PMID: 8806084

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PC-PLD2 Antibody (B-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PC-PLD2 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PC-PLD2 Antibody (B-3) Specifications

Alternate Product Name: Phospholipase D2

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 820-846

Epitope Target: PC-PLD2

Epitope Species: human

Product Reactivity/Detection: isoforms PC-PLD2A and PC-PLD2B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PC-PLD2 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin