Pax-6 Antibody (AD2.38): sc-32766

Pax-6 Antibody (AD2.38) is a mouse monoclonal IgG1 antibody that detects Pax-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pax-6 antibody (AD2.38) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pax-6 plays a crucial role in eye development, functioning as a transcriptional regulator that binds to specific DNA sequences to control the expression of genes essential for the formation of ocular structures. Mutations or deletions in the Pax-6 gene can lead to severe congenital eye disorders, including aniridia, characterized by the absence of the iris, and Peters anomaly, which involves corneal clouding and other anterior segment malformations. The structure of Pax-6 includes a paired domain composed of two distinct DNA-binding subdomains, which allows effective recognition and binding to target genes, thereby influencing the developmental pathways critical for eye formation. The human Pax-6 gene produces two alternatively spliced isoforms, each with unique structural features that contribute to regulatory functions during embryonic development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Pax-6 Antibody (AD2.38) References:

1. Pax-6 is required for thalamocortical pathway formation in fetal rats.  |  Kawano, H., et al. 1999. J Comp Neurol. 408: 147-60. PMID: 10333267
2. Pax-6 regulates expression of SFRP-2 and Wnt-7b in the developing CNS.  |  Kim, AS., et al. 2001. J Neurosci. 21: RC132. PMID: 11222670
3. The role of Pax-6 in lens regeneration.  |  Madhavan, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 14848-53. PMID: 17003134
4. Pax-6 is expressed early in the differentiation of a corneal epithelial model system.  |  García-Villegas, R., et al. 2009. J Cell Physiol. 220: 348-56. PMID: 19347868
5. Quantitation of Pax-6 protein in ocular impression cytology samples using an electrochemiluminescence immunoassay.  |  Zaworski, PG., et al. 2022. Anal Biochem. 656: 114876. PMID: 36058293
6. The role of Pax-6 in eye and nasal development.  |  Grindley, JC., et al. 1995. Development. 121: 1433-42. PMID: 7789273
7. The Pax-6 homeobox gene is expressed throughout the corneal and conjunctival epithelia.  |  Koroma, BM., et al. 1997. Invest Ophthalmol Vis Sci. 38: 108-20. PMID: 9008636
8. Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain.  |  Mastick, GS., et al. 1997. Development. 124: 1985-97. PMID: 9169845
9. Pax-6 is involved in the specification of hindbrain motor neuron subtype.  |  Osumi, N., et al. 1997. Development. 124: 2961-72. PMID: 9247338
10. Pax-6 and Cdx-2/3 interact to activate glucagon gene expression on the G1 control element.  |  Ritz-Laser, B., et al. 1999. J Biol Chem. 274: 4124-32. PMID: 9933606

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-6 Antibody (AD2.38) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-6 Antibody (AD2.38) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-6 Antibody (AD2.38) Specifications

Alternate Product Name: Pax-6

Product Clone Name: AD2.38

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 1-206

Epitope Target: Pax-6

Epitope Species: human

Product Reactivity/Detection: Pax-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for Pax-6 Antibody (AD2.38): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Pax-6 antibody is Pax-6 Antibody (PAX6): sc-81649.