Pax-6 Antibody (PAX6): sc-81649

Pax-6 Antibody (PAX6) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Pax-6 protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pax-6 antibody (PAX6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pax-6 plays a crucial role in eye development and is essential for the formation of the eye′s anterior segment, including the cornea and iris. Pax-6 function is vital, as mutations in the Pax-6 gene can lead to severe congenital eye disorders, such as aniridia, characterized by the absence of the iris, which can result in significant vision impairment or blindness. Pax-6 is involved in other anterior segment malformations, including Peters anomaly, which is marked by corneal clouding and various adhesions affecting the eye′s structures. Pax-6 gene encodes a transcriptional regulator that binds to specific DNA sequences through its paired-type DNA-binding domain, which consists of two subdomains that work together to regulate gene expression critical for eye development. Human Pax-6 gene produces two alternatively spliced isoforms, each with distinct structural features that contribute to its regulatory functions.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Pax-6 Antibody (PAX6) References:

1. Missense mutation in the alternative splice region of the PAX6 gene in eye anomalies.  |  Azuma, N., et al. 1999. Am J Hum Genet. 65: 656-63. PMID: 10441571
2. Sox-2 is expressed by glial and progenitor cells and Pax-6 is expressed by neuroblasts in the human subventricular zone.  |  Baer, K., et al. 2007. Exp Neurol. 204: 828-31. PMID: 17291498
3. Eye development under the control of SRp55/B52-mediated alternative splicing of eyeless.  |  Fic, W., et al. 2007. PLoS One. 2: e253. PMID: 17327915
4. Protein phosphatase-1 modulates the function of Pax-6, a transcription factor controlling brain and eye development.  |  Yan, Q., et al. 2007. J Biol Chem. 282: 13954-65. PMID: 17374606
5. Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes.  |  Xu, H., et al. 2007. Invest Ophthalmol Vis Sci. 48: 1674-82. PMID: 17389499
6. Mutation analysis of gene PAX6 in human gliomas.  |  Pinto, GR., et al. 2007. Genet Mol Res. 6: 1019-25. PMID: 18273794
7. PAX6 analysis of two unrelated families from the Arabian Peninsula with classic hereditary aniridia.  |  Khan, AO. and Aldahmesh, MA. 2008. Ophthalmic Genet. 29: 145-8. PMID: 18766996
8. Expression of pax-6 during urodele eye development and lens regeneration.  |  Del Rio-Tsonis, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 5092-6. PMID: 7761453
9. Mutations at the PAX6 locus are found in heterogeneous anterior segment malformations including Peters' anomaly.  |  Hanson, IM., et al. 1994. Nat Genet. 6: 168-73. PMID: 8162071
10. PAX6 mutations in aniridia.  |  Hanson, IM., et al. 1993. Hum Mol Genet. 2: 915-20. PMID: 8364574
11. Pax-6 and alphaB-crystallin/small heat shock protein gene regulation in the murine lens. Interaction with the lens-specific regions, LSR1 and LSR2.  |  Gopal-Srivastava, R., et al. 1996. J Biol Chem. 271: 23029-36. PMID: 8798491
12. Regulation of PAX-6 gene transcription: alternate promoter usage in human brain.  |  Okladnova, O., et al. 1998. Brain Res Mol Brain Res. 60: 177-92. PMID: 9757029

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-6 Antibody (PAX6) has 65 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-6 Antibody (PAX6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-6 Antibody (PAX6) Specifications

Alternate Product Name: Pax-6

Product Clone Name: PAX6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-223

Epitope Target: Pax-6

Epitope Species: chicken

Product Reactivity/Detection: Pax-6

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 65 citations in various scientific publications

Available Conjugates/Forms for Pax-6 Antibody (PAX6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin