PAR-2 Antibody (3G233): sc-71842

PAR-2 Antibody (3G233) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Proteinase-Activated Receptor 2 (PAR-2), also known as F2RL1 or PAR2, across mouse, rat, and human species using applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). PAR-2 monoclonal antibody (3G233) targets an epitope within amino acids 37-50 of the human PAR-2 protein, ensuring accurate identification and analysis in various experimental contexts. PAR-2 functions as a G protein-coupled receptor that plays a crucial role in mediating inflammatory responses, pain perception, and cellular proliferation by being activated through proteolytic cleavage by enzymes like trypsin, which is essential for understanding inflammatory diseases and developing potential therapeutic interventions. Located on the cell surface, PAR-2 facilitates the transduction of extracellular signals into intracellular responses, which is vital for processes such as tissue repair and immune modulation. Structurally, PAR-2 is characterized by its seven transmembrane domains, allowing interaction effectively with G proteins and initiate downstream signaling pathways, making its structure integral to its function. PAR-2 undergoes post-translational modifications, including phosphorylation, which regulate signaling activity and receptor desensitization, thereby modulating cellular responses to various stimuli. Additionally, PAR-2 interacts with specific proteins such as beta-arrestins, which are involved in receptor internalization and signaling regulation, and PDZ domain-containing proteins that influence cellular localization and functional interactions. These interactions are critical for elucidating the mechanisms underlying PAR-2-mediated signaling pathways and their implications in diseases like arthritis and cancer. Anti-PAR-2 antibody (3G233) is available in both non-conjugated and conjugated forms, including horseradish peroxidase (HRP) and fluorescent labels, providing versatile tools for researchers investigating the complex biology of PAR-2 and its role in health and disease.

The preferred PAR-2 antibody is PAR-2 Antibody (SAM11): sc-13504.