PANK1 Antibody (B-9): sc-390865
- PANK1 Antibody (B-9) is a mouse monoclonal IgG1 κ PANK1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 136-152 within an internal region of PANK1 of human origin
- PANK1 Antibody (B-9) is recommended for detection of PANK1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PANK1 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PANK1 Antibody (B-9) for WB applications. These reagents are now offered in bundles with PANK1 Antibody (B-9) (see ordering information below).
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PANK1 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PANK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PANK1 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The pantothenate kinase 1 (PANK1) protein, consisting of 598 amino acids, is crucial for regulating coenzyme A (CoA) levels within cells, as PANK1 catalyzes the first step in CoA biosynthesis. CoA is vital for numerous metabolic pathways, including fatty acid synthesis and degradation, the citric acid cycle, and metabolism of carbohydrates and amino acids. PANK1 is primarily localized in the cytoplasm and is subject to inhibition by acetyl-CoA and manyl-CoA, as well as by high concentrations of non-esterified CoA (CoASH), which underscores PANK1′s role in maintaining cellular energy homeostasis. Notably, four isoforms of PANK1 arise from alternative splicing, with PANK1a and PANK1b being the catalytically active forms; PANK1a is predominantly expressed in tissues such as the brain, heart, kidney, liver, and skeletal muscle, while PANK1b is found at lower levels in kidney, liver, brain, and testis, and is absent in heart and skeletal muscle.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PANK1 Antibody (B-9) References:
- A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome. | Zhou, B., et al. 2001. Nat Genet. 28: 345-9. PMID: 11479594
- Cloning and characterization of a novel human pantothenate kinase gene. | Ni, X., et al. 2002. Int J Biochem Cell Biol. 34: 109-15. PMID: 11809413
- The murine pantothenate kinase (Pank1) gene encodes two differentially regulated pantothenate kinase isozymes. | Rock, CO., et al. 2002. Gene. 291: 35-43. PMID: 12095677
- PPARalpha controls the intracellular coenzyme A concentration via regulation of PANK1alpha gene expression. | Ramaswamy, G., et al. 2004. J Lipid Res. 45: 17-31. PMID: 14523052
- Plant coenzyme A biosynthesis: characterization of two pantothenate kinases from Arabidopsis. | Tilton, GB., et al. 2006. Plant Mol Biol. 61: 629-42. PMID: 16897480
- Crystal structures of human pantothenate kinases. Insights into allosteric regulation and mutations linked to a neurodegeneration disorder. | Hong, BS., et al. 2007. J Biol Chem. 282: 27984-93. PMID: 17631502
- Pantothenate kinase-associated neurodegeneration: insights from a Drosophila model. | Wu, Z., et al. 2009. Hum Mol Genet. 18: 3659-72. PMID: 19602483
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PANK1 Antibody (B-9) | sc-390865 | 200 µg/ml | $322.00 | |||
PANK1 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-530293 | 200 µg Ab; 10 µg BP | $361.00 | |||
PANK1 Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-523716 | 200 µg Ab, 40 µg BP | $361.00 | |||
PANK1 Antibody (B-9): m-IgG1 BP-HRP Bundle | sc-543976 | 200 µg Ab; 20 µg BP | $361.00 | |||
PANK1 Antibody (B-9) AC | sc-390865 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PANK1 Antibody (B-9) HRP | sc-390865 HRP | 200 µg/ml | $322.00 | |||
PANK1 Antibody (B-9) FITC | sc-390865 FITC | 200 µg/ml | $336.00 | |||
PANK1 Antibody (B-9) PE | sc-390865 PE | 200 µg/ml | $349.00 | |||
PANK1 Antibody (B-9) Alexa Fluor® 488 | sc-390865 AF488 | 200 µg/ml | $364.00 | |||
PANK1 Antibody (B-9) Alexa Fluor® 546 | sc-390865 AF546 | 200 µg/ml | $364.00 | |||
PANK1 Antibody (B-9) Alexa Fluor® 594 | sc-390865 AF594 | 200 µg/ml | $364.00 | |||
PANK1 Antibody (B-9) Alexa Fluor® 647 | sc-390865 AF647 | 200 µg/ml | $364.00 | |||
PANK1 Antibody (B-9) Alexa Fluor® 680 | sc-390865 AF680 | 200 µg/ml | $364.00 | |||
PANK1 Antibody (B-9) Alexa Fluor® 790 | sc-390865 AF790 | 200 µg/ml | $364.00 | |||
PANK1 (B-9) Neutralizing Peptide | sc-390865 P | 100 µg/0.5 ml | $69.00 |