PAM Antibody (F-4): sc-514110
- PAM Antibody (F-4) is a mouse monoclonal IgG1 κ PAM antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 501-800 mapping near the C-terminus of PAM of human origin
- PAM Antibody (F-4) is recommended for detection of PAM of human origin by WB, IP, IF and ELISA
- Anti-PAM Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PAM Antibody (F-4) for WB applications. These reagents are now offered in bundles with PAM Antibody (F-4) (see ordering information below).
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PAM Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PAM protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PAM (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Peptidylglycine alpha-amidating monooxygenase (PAM) plays a crucial role in the biosynthesis of bioactive alpha-amidated peptides, which are essential for numerous physiological processes, including hormone regulation and neurotransmission. This bifunctional enzyme catalyzes the conversion of glycine-extended precursors into their active forms, thereby influencing the activity of various neuropeptides and hormones. PAM′s function is highlighted by tissue-specific expression, particularly in the ovary, testis, lung, heart septum, anterior pituitary, and hypothalamus, where PAM contributes to the modulation of critical signaling pathways. PAM undergoes complex post-translational modifications, including phosphorylation, which are vital for proper localization and function within the cell. PAM′s cytosolic domain contains multiple signals that dictate subcellular localization, and PAM interacts with PAM cytosolic interactor proteins (P-CIPs), such as P-CIP2, which phosphorylates PAM at serine 949, further emphasizing the intricate regulatory mechanisms governing PAM′s activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PAM Antibody (F-4) References:
- The novel kinase peptidylglycine alpha-amidating monooxygenase cytosolic interactor protein 2 interacts with the cytosolic routing determinants of the peptide processing enzyme peptidylglycine alpha-amidating monooxygenase. | Caldwell, BD., et al. 1999. J Biol Chem. 274: 34646-56. PMID: 10574929
- Characterization and regulation of peptidylglycine alpha-amidating monooxygenase (PAM) expression in H9c2 cardiac myoblasts. | Girard, B., et al. 1999. Cell Tissue Res. 298: 489-97. PMID: 10639739
- Use of endoproteases to identify catalytic domains, linker regions, and functional interactions in soluble peptidylglycine alpha-amidating monooxygenase. | Husten, EJ., et al. 1993. J Biol Chem. 268: 9709-17. PMID: 8486658
- Phosphorylation of the cytosolic domain of peptidylglycine alpha-amidating monooxygenase. | Yun, HY., et al. 1995. J Biol Chem. 270: 30075-83. PMID: 8530412
- Distribution and processing of peptidylglycine alpha-amidating monooxygenase activity in rat dorsal root ganglia and sciatic nerves. | Takasugi, H., et al. 1996. Neurochem Int. 29: 397-403. PMID: 8939448
- Estrogen regulation of peptidylglycine alpha-amidating monooxygenase expression in anterior pituitary gland. | el Meskini, R., et al. 1997. Endocrinology. 138: 379-88. PMID: 8977427
- Identification of a novel cis-element in the 3'-untranslated region of mammalian peptidylglycine alpha-amidating monooxygenase messenger ribonucleic acid. | Fraboulet, S., et al. 1998. Endocrinology. 139: 894-904. PMID: 9492018
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAM Antibody (F-4) | sc-514110 | 200 µg/ml | $322.00 | |||
PAM Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-531033 | 200 µg Ab; 10 µg BP | $361.00 | |||
PAM Antibody (F-4): m-IgGκ BP-HRP Bundle | sc-524610 | 200 µg Ab, 40 µg BP | $361.00 | |||
PAM Antibody (F-4): m-IgG1 BP-HRP Bundle | sc-544369 | 200 µg Ab; 20 µg BP | $361.00 | |||
PAM Antibody (F-4) AC | sc-514110 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PAM Antibody (F-4) HRP | sc-514110 HRP | 200 µg/ml | $322.00 | |||
PAM Antibody (F-4) FITC | sc-514110 FITC | 200 µg/ml | $336.00 | |||
PAM Antibody (F-4) PE | sc-514110 PE | 200 µg/ml | $349.00 | |||
PAM Antibody (F-4) Alexa Fluor® 488 | sc-514110 AF488 | 200 µg/ml | $364.00 | |||
PAM Antibody (F-4) Alexa Fluor® 546 | sc-514110 AF546 | 200 µg/ml | $364.00 | |||
PAM Antibody (F-4) Alexa Fluor® 594 | sc-514110 AF594 | 200 µg/ml | $364.00 | |||
PAM Antibody (F-4) Alexa Fluor® 647 | sc-514110 AF647 | 200 µg/ml | $364.00 | |||
PAM Antibody (F-4) Alexa Fluor® 680 | sc-514110 AF680 | 200 µg/ml | $364.00 | |||
PAM Antibody (F-4) Alexa Fluor® 790 | sc-514110 AF790 | 200 µg/ml | $364.00 |