PACSIN3 Antibody (C-3): sc-166923

PACSIN3 Antibody (C-3) is a mouse monoclonal IgG2a antibody that detects PACSIN3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PACSIN3, or Protein Kinase C and Casein Kinase Substrate in Neurons 3, plays a crucial role in the regulation of vesicle formation and transport within the cytoplasm, which is essential for maintaining cellular communication and function. PACSIN3 contains a short proline-rich region and lacks the asparagine-proline-phenylalanine motifs found in other PACSIN family members, which may influence specific interactions and functional roles. Sequence analysis reveals that human PACSIN3 consists of 424 amino acids and is 94% identical to its mouse counterpart, highlighting the evolutionary conservation of function. Studies indicate that PACSIN3 is predominantly expressed in the lung, skeletal muscle, heart, brain, kidney, and uterus of mice, suggesting involvement in various physiological processes across different tissues. PACSIN3′s ability to oligomerize and bind to proteins such as dynamin, synaptojanin 1, and N-WASP through Src homology 3 domains emphasizes importance in cellular dynamics and vesicle trafficking. Anti-PACSIN3 antibody (C-3) serves as an invaluable tool for researchers investigating the intricate mechanisms of cellular transport and signaling pathways.

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PACSIN3 Antibody (C-3) References:

1. PACSIN3 binds ADAM12/meltrin alpha and up-regulates ectodomain shedding of heparin-binding epidermal growth factor-like growth factor.  |  Mori, S., et al. 2003. J Biol Chem. 278: 46029-34. PMID: 12952982
2. PACSIN3 overexpression increases adipocyte glucose transport through GLUT1.  |  Roach, W. and Plomann, M. 2007. Biochem Biophys Res Commun. 355: 745-50. PMID: 17320047
3. Phosphatidylinositol-4,5-biphosphate-dependent rearrangement of TRPV4 cytosolic tails enables channel activation by physiological stimuli.  |  Garcia-Elias, A., et al. 2013. Proc Natl Acad Sci U S A. 110: 9553-8. PMID: 23690576
4. Temporal endogenous gene expression profiles in response to lipid-mediated transfection.  |  Martin, TM., et al. 2015. J Gene Med. 17: 14-32. PMID: 25663588
5. Structural Basis of TRPV4 N Terminus Interaction with Syndapin/PACSIN1-3 and PIP2.  |  Goretzki, B., et al. 2018. Structure. 26: 1583-1593.e5. PMID: 30244966
6. Homozygous TRPV4 mutation causes congenital distal spinal muscular atrophy and arthrogryposis.  |  Velilla, J., et al. 2019. Neurol Genet. 5: e312. PMID: 31041394
7. Interleukin-6 affects pacsin3, ephrinA4 expression and cytoskeletal proteins in differentiating primary skeletal myoblasts through transcriptional and post-transcriptional mechanisms.  |  Milewska, M., et al. 2020. Cell Tissue Res. 380: 155-172. PMID: 31820147
8. The mechanosensitive Piezo1 channel controls endosome trafficking for an efficient cytokinetic abscission.  |  Carrillo-Garcia, J., et al. 2021. Sci Adv. 7: eabi7785. PMID: 34714681
9. RNA sequencing and bioinformatics analysis revealed PACSIN3 as a potential novel biomarker for platinum resistance in epithelial ovarian cancer.  |  Han, GH., et al. 2022. J Gene Med. 24: e3452. PMID: 36170157
10. Biallelic truncating variants in PACSIN3 cause childhood-onset myopathy with hyperCKaemia.  |  Distelmaier, F., et al. 2024. Brain. 147: e45-e49. PMID: 38637313

The preferred PACSIN3 antibody is PACSIN3 Antibody (F-8): sc-373952.