p35 Antibody (G-7): sc-518015

p35 Antibody (G-7) is a mouse monoclonal IgG2a antibody that detects p35 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p35 plays a crucial role as a regulatory subunit of cyclin-dependent kinase-5 (Cdk5), which is essential for various neuronal functions, including neuronal development, synaptic plasticity, and the regulation of the cell cycle in post-mitotic neurons. p35 (G-7) antibody enables researchers to study p35′s ability to activate Cdk5, thereby influencing the kinase′s activity and downstream signaling pathways. p35 is expressed predominantly in the brain, where p35 forms a complex with Cdk5, facilitating enzymatic function. In contrast, a truncated form of p35, known as p25, is found in various tissues and can lead to aberrant Cdk5 activity, which has been implicated in neurodegenerative diseases. anti-p35 antibody (G-7) serves as a valuable tool for studying the dynamics of Cdk5 regulation and implications in neuronal health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p35 Antibody (G-7) References:

1. Targeted expression of baculovirus p35 caspase inhibitor in oligodendrocytes protects mice against autoimmune-mediated demyelination.  |  Hisahara, S., et al. 2000. EMBO J. 19: 341-8. PMID: 10654933
2. Detection of immunoglobulin M antibodies to P35 antigen of Toxoplasma gondii for serodiagnosis of recently acquired infection in pregnant women.  |  Suzuki, Y., et al. 2000. J Clin Microbiol. 38: 3967-70. PMID: 11060053
3. Opsonic function and concentration of human serum ficolin/P35.  |  Taira, S., et al. 2000. Fukushima J Med Sci. 46: 13-23. PMID: 11446374
4. Fas engagement induces neurite growth through ERK activation and p35 upregulation.  |  Desbarats, J., et al. 2003. Nat Cell Biol. 5: 118-25. PMID: 12545171
5. Cyclin-dependent kinase 5 activator p35 over-expression and amyloid beta synergism increase apoptosis in cultured neuronal cells.  |  Utreras, E., et al. 2009. Neuroscience. 161: 978-87. PMID: 19362124
6. Contribution of interleukin-12 p35 (IL-12p35) and IL-12p40 to protective immunity and pathology in mice infected with Chlamydia muridarum.  |  Chen, L., et al. 2013. Infect Immun. 81: 2962-71. PMID: 23753624
7. Anti-IL-23 receptor monoclonal antibody prevents CD4+ T cell-mediated colitis in association with decreased systemic Th1 and Th17 responses.  |  Imamura, E., et al. 2018. Eur J Pharmacol. 824: 163-169. PMID: 29391156
8. Phage display antibodies against ectromelia virus that neutralize variola virus: Selection and implementation for p35 neutralizing epitope mapping.  |  Khlusevich, Y., et al. 2018. Antiviral Res. 152: 18-25. PMID: 29427674
9. An improved luciferase immunosorbent assay for ultrasensitive detection of antibodies against African swine fever virus.  |  Wang, Q., et al. 2022. Front Microbiol. 13: 1013678. PMID: 36246209
10. Evidence for the participation of the neuron-specific CDK5 activator P35 during laminin-enhanced axonal growth.  |  Paglini, G., et al. 1998. J Neurosci. 18: 9858-69. PMID: 9822744

The preferred p35 antibody is p35 Antibody (B-1): sc-518009.