Anti-p300 Antibody (F-4) is a mouse monoclonal IgG2a κ p300 antibody, cited in 56 publications, provided at 200 µg/ml
raised against amino acids 774-1045 of p300 of human origin
Anti-p300 Antibody (F-4) is recommended for detection of p300 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-p300 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-48343 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p300 (F-4): sc-48343.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for p300 Antibody (F-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with p300 Antibody (F-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
p300 Antibody (F-4) is a high quality monoclonal p300 antibody (also designated KAT3B antibody or EP300 antibody) suitable for the detection of the p300 protein of mouse, rat and human origin. p300 Antibody (F-4) is available as both the non-conjugated anti-p300 antibody form, as well as multiple conjugated forms of anti-p300 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences